Abstract

In the present study, gold nanoparticles (CRnp) has been prepared using aqueous extract of Cyperus rotundus root at room temperature, sunlight, sonication, microwave oven heating and hot air oven method. Effect of concentration variation was studied using microwave-assisted synthesis. Grayish pink colour gold nanoparticles were formed within 5 s in microwave-assisted synthesis. Preliminary phytochemical screening showed the presence of alkaloids, protein and phenolic groups. These metabolites may be the responsible for the formation of gold nanoparticles. Reduced graphene oxide (CRrGO) was prepared using refluxing method and nano composite (CRNC) was prepared using equal ratio (1:1) of CRnp and CRrGO under homogenization method. The prepared CRnp, CRrGO and CRNC were characterized by spectroscopic techniques such as UV–visible spectroscopy, FTIR, XRD, Raman spectroscopy and FESEM. Thermal stability analysis of the CRrGO was carried out using TGA. FESEM results revealed the synthesized CRnp to be spherical in nature with average diameter 15 nm. CRrGO showed flake-like structures with few layers which was further confirmed by FESEM and Raman spectroscopy. The FESEM image of CRNC clearly portrays CRnp to be strongly bound to the surface of CRrGO. Anti-bacterial activity of the synthesized CRnp, CRrGO, CRNC and CRa synthesized nanoparticles to be active against gram negative and gram positive bacteria.

Graphic abstract

中文翻译：

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香附根提取物中金属和非金属纳米颗粒对引起病原的传染病的抗菌活性

摘要

在本研究中，使用香附子的水提取物制备了金纳米颗粒（CRnp）根在室温下，日光下，超声处理，微波炉加热和热风烤箱的方法。使用微波辅助合成研究了浓度变化的影响。微波辅助合成在5 s内形成了浅粉红色的金纳米颗粒。初步的植物化学筛选显示出生物碱，蛋白质和酚基团的存在。这些代谢物可能是金纳米颗粒形成的原因。采用回流法制备了还原型氧化石墨烯（CRrGO），并在均质化法下采用等比例（1：1）的CRnp和CRrGO制备了纳米复合材料（CRNC）。制备的CRnp，CRrGO和CRNC通过光谱技术进行表征，例如紫外可见光谱，FTIR，XRD，拉曼光谱和FESEM。使用TGA对CRrGO进行热稳定性分析。FESEM结果表明，合成的CRnp本质上是球形的，平均直径为15 nm。CRrGO显示出具有很少层的片状结构，这通过FESEM和拉曼光谱进一步证实。CRNC的FESEM图像清楚地描绘了CRnp与CRrGO的表面牢固结合。合成的CRnp，CRrGO，CRNC和CRa合成的纳米颗粒的抗菌活性对革兰氏阴性和革兰氏阳性细菌具有活性。

图形摘要