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Antimicrobial activity of metal and non-metallic nanoparticles from Cyperus rotundus root extract on infectious disease causing pathogens
Journal of Plant Biochemistry and Biotechnology ( IF 1.6 ) Pub Date : 2019-08-05 , DOI: 10.1007/s13562-019-00523-1 Santhiya Sasidharan , Lalitha Pottail
中文翻译:
香附根提取物中金属和非金属纳米颗粒对引起病原的传染病的抗菌活性
更新日期:2019-08-05
Journal of Plant Biochemistry and Biotechnology ( IF 1.6 ) Pub Date : 2019-08-05 , DOI: 10.1007/s13562-019-00523-1 Santhiya Sasidharan , Lalitha Pottail
Abstract
In the present study, gold nanoparticles (CRnp) has been prepared using aqueous extract of Cyperus rotundus root at room temperature, sunlight, sonication, microwave oven heating and hot air oven method. Effect of concentration variation was studied using microwave-assisted synthesis. Grayish pink colour gold nanoparticles were formed within 5 s in microwave-assisted synthesis. Preliminary phytochemical screening showed the presence of alkaloids, protein and phenolic groups. These metabolites may be the responsible for the formation of gold nanoparticles. Reduced graphene oxide (CRrGO) was prepared using refluxing method and nano composite (CRNC) was prepared using equal ratio (1:1) of CRnp and CRrGO under homogenization method. The prepared CRnp, CRrGO and CRNC were characterized by spectroscopic techniques such as UV–visible spectroscopy, FTIR, XRD, Raman spectroscopy and FESEM. Thermal stability analysis of the CRrGO was carried out using TGA. FESEM results revealed the synthesized CRnp to be spherical in nature with average diameter 15 nm. CRrGO showed flake-like structures with few layers which was further confirmed by FESEM and Raman spectroscopy. The FESEM image of CRNC clearly portrays CRnp to be strongly bound to the surface of CRrGO. Anti-bacterial activity of the synthesized CRnp, CRrGO, CRNC and CRa synthesized nanoparticles to be active against gram negative and gram positive bacteria.Graphic abstract
中文翻译:
香附根提取物中金属和非金属纳米颗粒对引起病原的传染病的抗菌活性