RNA-guided CRISPR/Cas9 technology has been developed for gene/genome editing (GE) in organisms across kingdoms. However, in planta delivery of the two core GE components, Cas9 and small guide RNA (sgRNA), often involves time-consuming and labor-intensive production of transgenic plants. Here we show that Foxtail mosaic virus, a monocot- and dicot-infecting potexvirus, can simultaneously express Cas9, sgRNA, and an RNAi suppressor to efficiently induce GE in Nicotiana benthamiana through a transgenic plant-free manner.

中文翻译：

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RNAi 抑制因子在植物病毒介导的基因编辑中激活。


RNA 引导的 CRISPR/Cas9 技术已开发用于跨界生物体的基因/基因组编辑 (GE)。然而，在植物中传递两个核心 GE 成分 Cas9 和小向导 RNA (sgRNA)，通常涉及转基因植物的耗时和劳动密集型生产。在这里，我们证明Foxtail 花叶病毒（一种感染单子叶植物和双子叶植物的 Potexvirus）可以同时表达 Cas9、sgRNA 和 RNAi 抑制子，通过无转基因植物的方式在本塞姆氏烟草中有效诱导 GE。