Abstract

Strain B-13176, which manifests antibacterial and fungicidal activity, has been isolated via the screening of spore-forming bacteria and identified as Bacillus pumilus based on an analysis of variable regions of 16S RNA sequences. The cultural and morphological characteristics of the strain were studied, the dynamics of the synthesis of fungicidal and antibacterial metabolites and their location in culture were determined, and their resistance to physicochemical factors was investigated. It was shown that the strain possesses a pronounced activity against Aspergillus niger fungi and methycillin-resistant Staphylococcus aureus (MRSA) bacteria. The culture liquid of the strain was fractioned via centrifugation, ultrafiltration, extraction, and high-performance liquid chromatography (HPLC). Mass-spectrometry was used to show that the Bacillus pumilus B-13176 strain produces active, thermostable, protease-resistant metabolites of peptide origin that are located in the CL pellet (the fungicidal component) and supernatant (the antibacterial component).

中文翻译：

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短小芽孢杆菌B-13176及其代谢产物对黑曲霉和金黄色葡萄球菌（MRSA）具有杀真菌和抗菌活性的研究

摘要

通过对孢子形成细菌的筛选，已分离出具有抗菌和杀真菌活性的B-13176菌株，并根据16S RNA序列可变区的分析将其鉴定为短小芽孢杆菌。研究了该菌株的培养和形态特征，确定了杀真菌和抗菌代谢产物的合成动力学及其在培养物中的位置，并研究了其对理化因子的抗性。结果表明，该菌株对黑曲霉真菌和耐甲氧西林金黄色葡萄球菌具有明显的活性。（MRSA）细菌。通过离心，超滤，提取和高效液相色谱法（HPLC）分离菌株的培养液。质谱分析表明，短小芽孢杆菌B-13176菌株产生的肽来源的活性，热稳定，耐蛋白酶的代谢产物位于CL颗粒（杀真菌成分）和上清液（抗菌成分）中。