Natural products are widely used due to their various biological activities which include antiinflammatory, antioxidant, and anti-obesity effects. In this study, we determined the antioxidative and anti-obesity effects of Polygonum cuspidatum 50% ethanol extract (PEE). The antioxidative effect of PEE was evaluated using its radical scavenging activity, total phenolic content, and reducing power. The anti-obesity effect of PEE was investigated using 3T3-L1 adipocytes. The antioxidative activity of PEE was progressively increased in various concentrations, mainly due to the presence of phenolic compounds. PEE also alleviated lipid accumulation on 3T3-L1 adipocytes and downregulated the mRNA and protein production of adipogenesis-related (SREBP-1c, PPARγ, C/EBPα) and lipogenesis-related (aP2, FAS, ACC) markers. Furthermore, we found that the inhibitory effect on lipid accumulation via PEE was caused by the alleviation of NF-κB, p38 MAPK, ERK1/2, and JNK at the protein level. Taken together, our results imply that PEE is a potential antioxidant that can prevent obesityassociated disorders.

中文翻译：

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虎杖提取物通过减轻 3T3-L1 脂肪细胞脂质积累的抗氧化和抗肥胖活性。

天然产物因其多种生物活性而被广泛使用，包括抗炎、抗氧化和抗肥胖作用。在这项研究中，我们确定了虎杖的抗氧化和抗肥胖作用50% 乙醇提取物 (PEE)。PEE 的抗氧化作用通过其自由基清除活性、总酚含量和还原能力进行评估。使用 3T3-L1 脂肪细胞研究了 PEE 的抗肥胖作用。PEE 的抗氧化活性在不同浓度下逐渐增加，这主要是由于酚类化合物的存在。PEE 还减轻了 3T3-L1 脂肪细胞上的脂质积累，并下调了脂肪生成相关（SREBP-1c、PPARγ、C/EBPα）和脂肪生成相关（aP2、FAS、ACC）标记物的 mRNA 和蛋白质生成。此外，我们发现 PEE 对脂质积累的抑制作用是由蛋白质水平的 NF-κB、p38 MAPK、ERK1/2 和 JNK 的减轻引起的。总之，我们的结果表明 PEE 是一种潜在的抗氧化剂，可以预防肥胖相关疾病。