Sex-determining region Y-box containing gene 30 (SOX30) is a newly identified tumor-associated gene in several types of cancer. However, whether SOX30 is involved in the development and progression of prostate cancer remains unknown. This study investigated the potential role of SOX30 in prostate cancer. Prostate cancer cell lines and a normal prostate epithelial cell line were used for the experiments. The expression of SOX30 was determined using quantitative real-time PCR and western blot analysis. The malignant cellular behaviors of prostate cancer were assessed using the Cell Counting Kit-8, colony formation and Matrigel invasion assays. The miRNA–mRNA interaction was validated using the dual-luciferase reporter assay. SOX30 expression was lower in cells of prostate cancer lines than in cells of the normal prostate epithelial line. Its overexpression repressed the proliferation and invasion of prostate cancer cells. SOX30 was identified as a target gene of microRNA-653-5p (miR-653-5p), which is upregulated in prostate cancer tissues. MiR-653-5p overexpression decreased SOX30 expression, while its inhibition increased SOX30 expression in prostate cancer cells. MiR-653-5p inhibition also markedly restricted prostate cancer cell proliferation and invasion. SOX30 overexpression or miR-653-5p inhibition significantly reduced β-catenin expression and downregulated the activation of Wnt/β-catenin signaling. SOX30 knockdown significantly reversed the miR-653-5p inhibition-mediated inhibitory effect on the proliferation, invasion and Wnt/β-catenin signaling in prostate cancer cells. These results reveal a tumor suppressive function for SOX30 in prostate cancer and confirmed the gene as a target of miR-653-5p. SOX30 upregulation due to miR-653-5p inhibition restricted the proliferation and invasion of prostate cancer cells, and this was associated with Wnt/β-catenin signaling suppression. These findings highlight the importance of the miR-653-5p–SOX30–Wnt/β-catenin signaling axis in prostate cancer progression.

中文翻译：

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SOX30是miR-653-5p的靶基因，通过抑制Wnt/β-catenin信号传导来抑制前列腺癌细胞的增殖和侵袭


含有基因 30 (SOX30) 的性别决定区 Y 盒是在多种癌症中新发现的肿瘤相关基因。然而，SOX30是否参与前列腺癌的发生和进展仍不清楚。这项研究调查了 SOX30 在前列腺癌中的潜在作用。前列腺癌细胞系和正常前列腺上皮细胞系用于实验。使用定量实时PCR和蛋白质印迹分析测定SOX30的表达。使用细胞计数试剂盒-8、集落形成和基质胶侵袭测定来评估前列腺癌的恶性细胞行为。使用双荧光素酶报告基因测定验证 miRNA-mRNA 相互作用。前列腺癌细胞系细胞中的 SOX30 表达低于正常前列腺上皮系细胞中的表达。它的过度表达抑制了前列腺癌细胞的增殖和侵袭。 SOX30 被确定为 microRNA-653-5p (miR-653-5p) 的靶基因，该基因在前列腺癌组织中表达上调。 miR-653-5p 过表达会降低前列腺癌细胞中 SOX30 的表达，而其抑制会增加 SOX30 的表达。 miR-653-5p 抑制也显着限制了前列腺癌细胞的增殖和侵袭。 SOX30 过表达或 miR-653-5p 抑制显着降低 β-catenin 表达并下调 Wn​​t/β-catenin 信号传导的激活。 SOX30 敲低显着逆转了 miR-653-5p 抑制介导的对前列腺癌细胞增殖、侵袭和 Wnt/β-catenin 信号传导的抑制作用。这些结果揭示了 SOX30 在前列腺癌中的肿瘤抑制功能，并证实该基因是 miR-653-5p 的靶标。 miR-653-5p 抑制导致 SOX30 上调，限制了前列腺癌细胞的增殖和侵袭，这与 Wnt/β-catenin 信号传导抑制有关。这些发现强调了 miR-653-5p–SOX30–Wnt/β-catenin 信号轴在前列腺癌进展中的重要性。