Activity and properties of two phosphohydrolases, nucleoside diphosphatase (NDPase) and 5′-nucleotidase, were assayed in subcellular fractions of turbellaria Phagocata sibirica Sabussov (Turbellaria, Planariidae). The highest activity of N D Pase was observed in microsomes while that of 5′-nucleotidase in mitochondria and cytosolic fractions (12 000 and 105 000 g). The correlation between the rates of the relevant enzymatic reactions and concentrations of substrates and metal ions as well as the impact of various effectors and divalent cations (Mg2+, Mn2+, Ca2+, Zn2+, Cu2+) on phosphohydrolase activities were studied.

中文翻译：

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Turbellaria Phagocata sibirica 中的磷酸水解酶

在 turbellaria Phagocata sibirica Sabussov (Turbellaria, Planariidae) 的亚细胞级分中测定了两种磷酸水解酶、核苷二磷酸酶 (NDPase) 和 5'-核苷酸酶的活性和特性。在微粒体中观察到 ND Pase 的最高活性，而在线粒体和细胞质部分（12 000 和 105 000 g）中观察到 5'-核苷酸酶的活性。研究了相关酶促反应速率与底物和金属离子浓度之间的相关性，以及各种效应物和二价阳离子（Mg2+、Mn2+、Ca2+、Zn2+、Cu2+）对磷酸水解酶活性的影响。