Abstract

Two new polycyclic pyrroloquinazoline alkaloids with unprecedented skeleton, anisulcusines A (1) and B (2), along with four known compounds (3–6), were identified from the aerial parts of Anisotes trisulcus (Forssk.) Nees. To our knowledge, anisulcusines A and B are the first polycyclic pyrroloquinazoline alkaloids that possess a unique N-methyl-1,2-dihydro-1'H-spiro[benzo[d][1,3]oxazine moiety. The chemical structures of the new compounds were elucidated through extensive spectroscopic analyses and high-resolution mass spectroscopy. Anisulcusine B (2) exerted moderate cytotoxic effect on cultured human hepatoma (HuH7) cells, whereas compounds 1 and 3–5 exhibited mild cell proliferative or growth stimulatory activity.

• HIGHLIGHTS

• Two new polycyclic pyrroloquinazoline alkaloids from Anisotes trisulcus.

• Structures were elucidated on the basis of 1D- and 2D-NMR and HR-ESI-MS spectra.

• Compound (2) exerted moderate cytotoxic effect against human hepatoma (HuH7) cells.

• Compounds (1, 3–5) exhibited mild cell proliferative or growth stimulatory activity.

摘要

以前所未有的骨架两个新的多环pyrroloquinazoline生物碱，anisulcusines A（1）和B（2），具有四个已知化合物（沿3 - 6），从地上部分识别Anisotes trisulcus（Forssk）穿心莲。据我们所知，anisulcusines A和B是所述第一多环生物碱pyrroloquinazoline具有独特ñ -甲基-1,2-二氢-1' ħ -螺[苯并[ d ] [1,3]恶嗪部分。通过广泛的光谱分析和高分辨率质谱阐明了新化合物的化学结构。茴香氨酸B（2）对培养的人肝癌细胞（HuH7）具有中等程度的细胞毒性作用，而化合物1和3 – 5则表现出轻度的细胞增殖或生长刺激活性。

• 强调

• 两个新的多环吡咯并喹唑啉生物碱。

• 根据1D-和2D-NMR和HR-ESI-MS光谱阐明了结构。

• 化合物（2）对人肝癌（HuH7）细胞发挥中等的细胞毒性作用。

• 化合物（1，3 - 5）显示出温和的细胞增殖或生长刺激活性。