Advancements in health care monitoring demand a rapid, accurate and reliable early diagnosis of “Heart Attack” (acute myocardial infarction) with an objective to develop a cost-effective, rapid and label-free point of care diagnostic test kit for the detection of cardiac troponin I (cTnI) on paper-based multi-frequency impedimetric transducers. Paper based sensing platforms were developed by integrating carboxyl group functionalized multi-walled carbon nanotubes (MWCNT) with antibodies of cardiac troponin I (anti-cTnI) biomarker and was characterized using Electrochemical Impedance Spectroscopy (EIS). Various concentrations of cTnI with anti cTnI were studied as a function of impedance change. The suitability of the proposed immunosensor is demonstrated by spiking cTnI in blood serum samples. The limit of detection (LoD) and sensitivity of the proposed sensor was determined to be 0.05 ng/mL and 1.85 mΩ/ng/mL respectively, with a response time of ~1 min. The shelf life of the fabricated sensor was nearly 30 days. The rapid response, very low detection limit, and cost effectiveness offer a portable platform to detect cTnI in blood serum samples. The proposed immunosensor, therefore, offers an affordable healthcare diagnostic platform in resource limited areas.

中文翻译：

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基于纸张的即时点免疫传感器，用于阻抗检测心肌肌钙蛋白I生物标志物。

医疗保健监测的进步要求对“心脏病发作”（急性心肌梗塞）进行快速，准确和可靠的早期诊断，目的是开发一种经济高效，快速且无标签的即时诊断服务，以检测心脏纸质多频阻抗传感器上的肌钙蛋白I（cTnI）。通过将羧基官能化的多壁碳纳米管（MWCNT）与心肌肌钙蛋白I（抗cTnI）生物标志物的抗体整合在一起，开发了基于纸的传感平台，并使用电化学阻抗谱（EIS）对其进行了表征。研究了不同浓度的cTnI和抗cTnI作为阻抗变化的函数。通过在血清样品中掺入cTnI来证明所建议的免疫传感器的适用性。所提出的传感器的检出限（LoD）和灵敏度分别确定为0.05 ng / mL和1.85mΩ/ ng / mL，响应时间约为1分钟。制成的传感器的保质期将近30天。快速反应，极低的检测限和成本效益为检测血清样品中的cTnI提供了一个便携式平台。因此，提出的免疫传感器在资源有限的地区提供了负担得起的医疗诊断平台。