Boswellia Carterii, a well-known plant species used in Korean medicine, is particularly effective in anti-cancer and inflammatory diseases. Its gum created from white resin is known to have therapeutic effects. However, the study on the anti-cancer pathway is very limited in human pancreatic cancer cells. thus, we investigated the changes in overall oncologic gene expression when treated BC in AsPC-1 cells. In this paper, we aimed to see how BC can be used to decrease cell viability with full explanation of gene expression in AsPC-1. We used MTT, microarray, and qRT-PCR to confirm our thesis. Results showed that BC inhibited cell viability in AsPC-1 by more than two-fold, which implies that BC has cell cytotoxic effects. Also, BC induced early and late apoptosis when treated for 24h on the pancreatic cancer cells. Differentially expressed genes were analyzed by microarray for demonstrated apoptotic effects at the mRNA level. The number of genes were related to participation in the oxidation-reduction process or signaling pathways. The signal pathway was verified by western blotting mainly STAT3 but also JAK, Src, ERK, JNK, and Akt. The results prove that stress signaling such as cytokines were regulated by BC stimulation. Therefore, our findings support the show that differentially expressed genes in BC-treated represent anti- cancer effects in pancreatic cancer and this has the potential for further understanding of the entire mechanism of cancer and its treatment.

中文翻译：

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乳香提取物的抗癌活性改变胰腺癌细胞中应激功能基因的表达

乳香，韩国医学中使用的一种著名的植物物种，在抗癌和炎性疾病方面特别有效。由白色树脂制成的口香糖具有治疗作用。但是，关于抗癌途径的研究在人胰腺癌细胞中非常有限。因此，我们研究了AsPC-1细胞中处理BC时总体肿瘤基因表达的变化。在本文中，我们旨在了解如何利用BC降低细胞活力，并全面解释AsPC-1中的基因表达。我们使用MTT，微阵列和qRT-PCR来证实我们的论文。结果表明，BC抑制AsPC-1中的细胞活力超过两倍，这表明BC具有细胞毒性作用。同样，当在胰腺癌细胞上治疗24小时时，BC会诱导早期和晚期凋亡。通过微阵列分析差异表达的基因，以证明在mRNA水平具有凋亡作用。基因的数量与参与氧化还原过程或信号通路有关。信号通路已通过蛋白质印迹法主要是STAT3进行了验证，但也通过JAK，Src，ERK，JNK和Akt进行了验证。结果证明，应激信号如细胞因子受BC刺激调节。因此，我们的发现支持了这样的研究，即在BC处理中差异表达的基因代表了胰腺癌的抗癌作用，这有可能进一步了解癌症的整体机理及其治疗方法。信号通路已通过蛋白质印迹法主要是STAT3进行了验证，但也通过JAK，Src，ERK，JNK和Akt进行了验证。结果证明，应激信号如细胞因子受BC刺激调节。因此，我们的发现支持了这样的研究，即在BC处理中差异表达的基因代表了胰腺癌的抗癌作用，这有可能进一步了解癌症的整体机理及其治疗方法。信号通路已通过蛋白质印迹法主要是STAT3进行了验证，但也通过JAK，Src，ERK，JNK和Akt进行了验证。结果证明，应激信号如细胞因子受BC刺激调节。因此，我们的发现支持了这样的研究，即在BC处理中差异表达的基因代表了胰腺癌的抗癌作用，这有可能进一步了解癌症的整体机理及其治疗方法。