The study deals with the effect of low pressure dielectric barrier discharge (LPDBD) plasma and plasma activated water (PAW) produced with Ar, O₂ and Air on germination and growth in rapeseed. Although H₂O₂ concentration showed no significant changes, α‑amylase activity (germination inducer) significantly increased in seeds due to LPDBD plasma. In addition, the activity of SOD and CAT was significantly induced in seeds of rapeseed treated with PAW. Rapeseed plants grown from the LPDBD plasma treated seeds showed significant improvements in shoot characteristics, chlorophyll synthesis, total soluble protein and sugar concentration compared to controls. Interestingly, plasma treated plants also showed no significant variations of H₂O₂ in tissue(s) which is supported by the biochemical and molecular evidence(s) of antioxidant enzymes. Plants exhibited a significant increase in tissue APX and CAT activities along with BnAPX and BnCAT expression(s) in roots when seeds were treated with LPDBD Air/O₂ and PAW O₂. This suggests that LPDBD plasma might have been involved with elevated level of reactive oxygen species, which was tightly controlled through the upregulation of APX and CAT activities and thus trigger the growth and development in rapeseed plants. These findings reveal the role and mechanisms of LPDBD technique facilitating germination and growth in rapeseed plants.

中文翻译：

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LPDBD血浆和血浆活化水对油菜种子发芽和生长的影响

该研究处理了由Ar，O ₂和空气产生的低压电介质阻挡放电（LPDBD）等离子体和等离子体活化水（PAW）对油菜种子发芽和生长的影响。尽管H ₂ O ₂浓度没有明显变化，但由于LPDBD血浆，α-淀粉酶活性（发芽诱导剂）在种子中显着增加。另外，用PAW处理的油菜种子中SOD和CAT的活性被显着诱导。与对照相比，从LPDBD血浆处理过的种子中生长的油菜籽在枝条特性，叶绿素合成，总可溶性蛋白和糖浓度方面显示出显着改善。有趣的是，等离子体处理过的植物也没有显示出H ₂ O的显着变化在组织中的抗氧化剂酶的生化和分子证据支持₂。用LPDBD Air / O ₂和PAW O ₂处理种子后，植物的根部组织APX和CAT活性以及BnAPX和BnCAT表达显着增加。这表明LPDBD血浆可能与活性氧水平升高有关，该活性氧水平通过APX和CAT活性的上调而受到严格控制，从而触发了油菜籽植物的生长发育。这些发现揭示了LPDBD技术促进油菜籽发芽和生长的作用和机理。