Epigenome editing is a promising approach for both basic research and clinical application. With the convergence of techniques from different fields, regulating gene expression artificially becomes possible. From a clinical point of view, targeted epigenome editing by CRISPR/Cas9 of disease-related genes offers novel therapeutic avenues for many diseases. In this review, we summarize the EpiEffectors used in epigenome editing by CRISPR/Cas9, current applications of epigenome editing and progress made in this field. Moreover, application challenges such as off-target effects, inefficient delivery, stability and immunogenicity are discussed. In conclusion, epigenome editing by CRISPR/Cas9 has broad prospects in the clinic, and future work will promote the application of this technology.

中文翻译：

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在临床环境中通过CRISPR / Cas9编辑表观基因组：可能性和挑战。

表观基因组编辑是用于基础研究和临床应用的有前途的方法。随着不同领域技术的融合，人工调节基因表达成为可能。从临床角度看，CRISPR / Cas9对疾病相关基因的靶向表观基因组编辑为许多疾病提供了新的治疗途径。在这篇综述中，我们总结了CRISPR / Cas9在表观基因组编辑中使用的EpiEffectors，表观基因组编辑的当前应用以及该领域的进展。此外，讨论了诸如脱靶效应，低效递送，稳定性和免疫原性等应用挑战。总之，CRISPR / Cas9的表观基因组编辑在临床上具有广阔的前景，未来的工作将促进该技术的应用。