Aspergillus nidulans is a fungal model organism extensively used in genetic approaches. It may reproduce sexually and asexually, with a well‐defined parasexual cycle. The current paper demonstrates that the limitation of nitrogen source facilitates the production of A. nidulans's nonmeiotic recombinants directly from heterokaryons, without the recovery of the diploid phase. Heterokaryons formed between master strains were inoculated in sodium nitrate‐low (basal medium [BM]) and sodium nitrate‐rich media (minimal medium [MM]). All mitotic segregants produced by the heterokaryons were tested for their mitotic stability in the presence of benomyl, the haploidizing agent. Only mitotically stable haploid segregants were selected for subsequent analysis. Phenotypic analyses of such haploids favored the characterization of nonmeiotic recombinants. As the number of such recombinants was higher in BM than in MM, nitrogen limitation may have facilitated the isolation of nonmeiotic recombinants from heterokaryons by stimulating nuclear fusion still inside the heterokaryotic mycelium as a survival strategy.

中文翻译：

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氮源限制促进构巢曲霉非减数分裂重组体的产生

构巢曲霉是一种广泛用于遗传方法的真菌模型生物。它可以有性和无性繁殖，具有明确的准性循环。目前的论文表明，氮源的限制有利于直接从异核中生产构巢曲霉的非减数分裂重组体，而无需恢复二倍体相。将主菌株之间形成的异核接种在低硝酸钠（基础培养基 [BM]）和富含硝酸钠的培养基（最低培养基 [MM]）中。在单倍体化剂苯菌灵存在下，测试了由异核体产生的所有有丝分裂分离子的有丝分裂稳定性。仅选择有丝分裂稳定的单倍体分离子用于后续分析。这种单倍体的表型分析有利于非减数分裂重组体的表征。