ABSTRACT

Objective: In patients with periodontitis, identification of protozoans and evaluation of some bacteria and clinical parameters associated and assessment of scaling and root planing (SRP) impact on their detection.

Methods: Before and after SRP, subgingival microbiota was collected in two pathological and one healthy site from 30 periodontitis patients. One healthy site from 30 control patients was also sampled. The usual clinical periodontal parameters were recorded; microbial detection was determined by PCR hybridization system for bacteria and qPCR for protozoans.

Results: In periodontitis group, Trichomonas tenax and two subtypes of Entamoeba gingivalis (ST1 and a variant ST2) were detected in respectively 33.3%, 70% and 18.3% of pathological samples, and in 6.7%, 10% and 3.3% healthy samples. In control group, ST1 alone was found in 3.3% of individuals. ST1 was associated with Gingival Index, Clinical Attachment Level (p ≤ 0.03) and with the total bacterial count (p = 0.02). T. tenax alone was associated with P. gingivalis, T. denticola and E. nodatum (p ≤ 0,02). After therapy, only T. tenax detection decreased significantly (p = 0.004) and no association between the protozoan elimination and improvement of pathological sites was found.

Conclusions: Protozoans were associated with some clinical parameters and/or periodontopathogens in patients with periodontitis.

摘要

目的：在牙周炎患者中，对原生动物进行鉴定并评估某些细菌和相关的临床参数，并评估结垢和根部刨光（SRP）对他们的检测的影响。

方法：在SRP前后，从30例牙周炎患者的两个病理和一个健康部位收集龈下微生物群。还对30名对照患者的1个健康部位进行了采样。记录通常的临床牙周参数；通过细菌的PCR杂交系统和原生动物的qPCR确定微生物检测。

结果：在牙周炎组中，分别在33.3％，70％和18.3％的病理样本以及6.7％，10％和3.3％的健康样本中检出了毛滴虫和两种亚型的牙龈变形虫（ST1和ST2变体）。在对照组中，仅ST1存在于3.3％的个体中。ST1与牙龈指数，临床依恋水平（p≤0.03）和细菌总数（p = 0.02）相关。单独的T. tenax与齿龈假单胞菌，T。denticola和E. nodatum相关（p≤0,02）。治疗后，只有T. tenax 检测显着降低（p = 0.004），并且未发现原生动物消除与病理部位改善之间的关联。

结论：原生动物与牙周炎患者的某些临床参数和/或牙周病原体有关。