The fluorescent probe techniques have been widely applied. When the fluorescence probes are selectively located in nano-structures, the fluorescence properties are highly influenced by the environment. Here, we systematically studied the location-influenced fluorescence of AIEgens in the microphase-separated structures. The AIEgen tetraphenylethene (TPE) was doped into polystyrene-b-polyisoprene (PS-{tib}-PI). TPE was selectively located in the PS nanodomains. The TPE fluorenscence was affected by the structural relaxation of PS when investigated in a wide range of temperatures, including the glass transiton and secondary transiton. When TPE groups were selectively located in the PI nanodomains, the fluorenscence was affected by the glass transitons of PI and PS blocks. Amphiphilic TPE derivative was located at the interface of the assembly. The fluorescence emission was influenced by the main transition and secondary transiton of PS blocks, as well as the main transition of PI blocks. These results would give new understanding of the interrelation between fluorescence probes and the nanostructures.

荧光探针技术已被广泛应用。当荧光探针选择性地位于纳米结构中时，荧光特性会受到环境的极大影响。在这里，我们系统地研究了微相分离结构中AIEgens的位置影响荧光。将AIEgen四苯乙烯（TPE）掺杂到聚苯乙烯-b中-聚异戊二烯（PS- {tib} -PI）。TPE选择性地位于PS纳米域中。当在很宽的温度范围内进行研究时，TPE荧光会受到PS的结构弛豫的影响，包括玻璃转移子和二次转移子。当TPE基团选择性地位于PI纳米域中时，荧光受PI和PS嵌段的玻璃转移的影响。两亲TPE衍生物位于组件的界面。荧光发射受PS嵌段的主要转变和次级跃迁以及PI嵌段的主要转变影响。这些结果将使人们对荧光探针和纳米结构之间的相互关系有了新的认识。