Sugarcane is a crop of economic importance providing sugar and bioenergy resource which has a substantial contribution to the national GDP of India as well as other countries. Genomes of the several grasses have co-evolved which share large-scale synteny and genome collinearity; hence it is possible to employ EST-SSR markers of one grass species for genetic study of other related species. A set of sixty potentially informative EST-derived microsatellite (SSR) markers were employed to examine their cross-taxa transferability across the limit of taxonomic boundaries of different grass genomes. The high (96.6%) rate of SSRs cross-transferability observed in genus Saccharum followed by Erianthus (93.3%), Narenga (88.3%), Sclerostachya (86.3%), and Miscanthus (83.3%). Relatively lower rates of cross amplification were observed in other cereal crops including Sorghum bicolor, Pennisetum glaucum, Zea mays, Triticum aestivum, Oryza sativa, and Avena sativa. Cross-transferable EST-SSR markers were utilized to evaluate phylogenetic relationships among genus Saccharum, closely related genera of Saccharum complex, and other allied cereal grasses. The polymorphic information content values ranged from 0.35 to 0.95, with an average of 0.71. Such a high rates of cross-taxa transferability of the EST-SSRs make them a potent tool for genetic studies and comparative genomics across wide evolutionary distances within and across genera along with technologically simplicity and cost effectiveness. EST-SSRs will facilitate to devise potent tools for genetic improvement through advance molecular breeding of such crops for high grain productivity and better quality. They will especially be useful for the orphan grass species, which contribute a major part of the global food security.

中文翻译：

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甘蔗表达序列标签衍生的微卫星（EST-SSR）标记物在相关谷物草上的跨类群转移性

甘蔗是一种具有经济重要性的作物，提供糖和生物能源资源，对印度以及其他国家的国民生产总值做出了重大贡献。几种草的基因组共同进化，它们具有大范围的同构性和基因组共线性。因此，可以将一种草种的EST-SSR标记用于其他相关种的遗传研究。一组六十个潜在的信息丰富的EST衍生的微卫星（SSR）标记用于检查跨不同草基因组分类边界的跨类转移能力。固态继电器的高（96.6％）速率交叉转印在属观察到甘蔗接着蔗茅（93.3％），Narenga（88.3％），Sclerostachya（86.3％）和芒草（83.3％）。在其他谷物作物中观察到相对较低的交叉扩增率，其中双色高粱，狼尾草，玉米，小麦，水稻和燕麦。跨转让EST-SSR标记来评价之间的属亲缘关系甘蔗，密切相关的属甘蔗复合谷物和其他谷物谷物。多态信息含量值的范围从0.35到0.95，平均为0.71。EST-SSR的如此高的交叉单元转移率使其成为遗传研究和跨基因组内和跨谱系的广泛进化距离上的比较基因组学的强大工具，以及技术上的简便性和成本效益。EST-SSRs将通过先进的农作物分子育种来促进设计有效的遗传改良工具，从而提高谷物产量和质量。它们对于孤儿草特别有用，因为它们在全球粮食安全中发挥了重要作用。