The chronological development of procedures for determining the concentration of cholesterol in plasma, serum, and whole blood is presented in the review. It is stated that, since the correlation between the risk of development of cardiovascular diseases and the concentrations of total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in human blood has been established by numerous medical studies, procedures for the measurement of these parameters have been developed most actively. A brief overview of these procedures and the results of their comparative tests in medical examinations of patients are given. Classifications are also proposed for procedures that determining total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in blood. The mechanism of action of the chemical reactions taking place in these procedures, the advantages and disadvantages, and prioritization of the field of their application are considered. Promising directions in the development and improvement of procedures are mentioned, ensuring more accurate measurements of the blood cholesterol concentration, and alternative means of determining cardiovascular disease risk are discussed.

中文翻译：

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血液中胆固醇的测定。第三部分

本综述介绍了按时间顺序开发的测定血浆，血清和全血中胆固醇浓度的程序。据指出，由于通过许多医学研究已经确定了心血管疾病发展的风险与人血中总胆固醇，低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的浓度之间的相关性，这些参数中的最积极地被开发了。简要概述了这些程序及其在患者体检中的比较测试结果。还提出了用于确定血液中总胆固醇，低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的程序的分类。考虑了在这些程序中发生的化学反应的作用机理，优点和缺点以及其应用领域的优先级。提到了程序开发和改进中的有希望的方向，确保了血液胆固醇浓度的更准确测量，并讨论了确定心血管疾病风险的替代方法。