Diatoms are thought to provide about 40% of total global photosynthesis and diatoms of the genus Coscinodiscus are an important, sometimes dominant, cosmopolitan component of the marine diatom community. The oomycete parasitoid Lagenisma coscinodisci is widespread in the northern hemisphere on its hosts in the genus Coscinodiscus. Because of its potential ecological importance, it would be a suitable pathogen model to investigate plankton/parasite interactions, but the species cannot be cultivated on media without its host, so far. Thus, it was the aim of this study to explore the potential of dual culture of host and pathogen in the laboratory and to optimise cultivation to ensure a long-term cultivation of the pathogen. Here, we report successful cultivation of a single spore strain of L. coscinodisci (Isla), on several Coscinodiscus species and strains, as well as the establishment of a cultivation routine with Coscinodiscus granii (CGS1 and CG36), which enabled us to maintain the single spore strain for more than 3 years in 6 cm Petri dishes and 10 ml tissue culture flasks. This opens up the opportunity to study the processes and mechanism in plankton/parasitoid interactions under controlled conditions.

中文翻译：

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卵菌Lagenisma coscinodisci Drebes和Coscinodiscus硅藻的双重培养作为浮游生物/寄生虫相互作用的模型

硅藻被认为提供了全球总光合作用的40％，而球藻属的硅藻是海洋硅藻群落中重要的，有时是占主导地位的世界性成分。卵菌寄生的Lagenisma coscinodisci在北半球的Coscinodiscus属宿主中广泛分布。由于其潜在的生态重要性，将是研究浮游生物/寄生虫相互作用的合适病原体模型，但是到目前为止，该物种不能在没有宿主的情况下在培养基上进行培养。因此，本研究的目的是在实验室中探索宿主和病原体双重培养的潜力，并优化培养以确保病原体的长期培养。在这里，我们报告了单孢菌L. coscinodisci（Isla）的成功培养，对几种球菌属和菌株进行培养，并建立了与球菌属（CGS1和CG36）的培养程序，这使我们能够在6 cm培养皿和10 ml组织培养瓶中将单个孢子菌株维持3年以上。 。这为研究受控条件下浮游生物/类寄生虫相互作用的过程和机理提供了机会。