Agrobacterium vitis and some strains of A. tumefaciens and A. rhizogenes cause a dangerous chronic disease on grape plants—crown gall. Molecular-genetic methods were used in the work to identify and study the diversity of pathogenic agrobacteria that cause crown gall in the vineyards of Krasnodar krai. The research material was 76 samples of young and lignified tumor-like outgrowths of crown gall of grapes collected from plants in 14 vineyards of Krasnodar krai with different origins of planting stock (Italy, France, Austria, Serbia, Bulgaria, and Russia). In all studied samples, the presence of A. vitis was identified using the PGF/PGR test-system for DNA analysis. When studying the diversity of agrobacteria by the type of Ti plasmids contained therein, four groups were identified: containing octopine-type Ti plasmids (59.5%), octopine/vitopine (13.5%), vitopine (13.5%), and nopaline type (13.5%). The obtained data indicate that crown gall of grapes in the vineyards of Krasnodar krai is caused by agrobacteria A. vitis, mainly of the octopine type.

中文翻译：

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基于Ti质粒类型的克拉斯诺达尔边疆区葡萄园中发现的致病性农杆菌的PCR鉴定

葡萄土壤杆菌和一些根癌农杆菌和发根农杆菌会在葡萄植物上造成危险的慢性病-冠gall。在这项工作中使用了分子遗传学方法来鉴定和研究引起克拉斯诺达尔克莱地区葡萄园冠k病的致病性农杆菌的多样性。研究材料是从克拉斯诺达尔边疆区（Krasnodar krai）的14个葡萄园中收集的76种年轻的和木质化的类瘤状冠冠葡萄样品，这些葡萄园的种植来源不同（意大利，法国，奥地利，塞尔维亚，保加利亚和俄罗斯）。在所有研究的样品中，葡萄球菌的存在使用PGF / PGR测试系统对DNA进行鉴定。当根据其中包含的Ti质粒的类型研究农杆菌的多样性时，确定了四组：包含章鱼碱型Ti质粒（59.5％），章鱼碱/维托平（13.5％），章鱼碱（13.5％）和胭脂碱型（13.5） ％）。所获得的数据表明，在Krasnodar边区的葡萄园葡萄的冠瘿通过农杆菌引起A.葡萄主要章鱼碱型的。