In this paper a carbon ionic liquid electrode (CILE) was fabricated by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF₆) as binder. By using the CILE as basal electrode, the hemoglobin (Hb) molecule was immobilized on the surface of CILE with a sodium alginate (SA) hydrogel and SiO₂ nanoparticles organic-inorganic composite material. The direct electrochemical behaviors of Hb in the bionanocomposite film were further studied in a pH 7.0 Britton-Robinson (B-R) buffer solution. A pair of well-defined quasi-reversible cyclic voltammetric peaks of Hb was obtained on SA/nano-SiO₂/Hb/CILE with the formal potential (E^0’) at -0.355 V (vs. SCE), which was the characteristic of heme Fe(III)/Fe(II) redox couples. The formal potential of Hb Fe(III)/Fe(II) couple shifted negatively with increasing pH of solution with a slope of -45.2 mV/pH, which indicated that a one electron transfer accompanied with one proton transportation. The immobilized Hb showed good electrocatalytic manner to the reduction of trichloroacetic acid (TCA).

中文翻译：

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固定在藻酸钠和SiO 2纳米颗粒生物纳米复合膜上的血红蛋白的直接电化学在碳离子液体电极上

本文采用1-丁基-3-甲基咪唑六氟磷酸盐（BMIMPF ₆）的室温离子液体作为粘合剂制备了碳离子液体电极（CILE）。通过使用CILE作为基础电极，血红蛋白（Hb）分子被藻酸钠（SA）水凝胶和SiO ₂纳米颗粒有机-无机复合材料固定在CILE的表面。在pH 7.0的Britton-Robinson（BR）缓冲溶液中进一步研究了Hb在仿生复合膜中的直接电化学行为。在SA / nano-SiO ₂ / Hb / CILE上以-0.355 V（v vs.）的形式势（E ^0'）获得了一对明确定义的Hb准可逆循环伏安峰。。SCE），这是血红素Fe（III）/ Fe（II）氧化还原对的特征。Hb Fe（III）/ Fe（II）的形式势随溶液pH的增加而负移，其斜率为-45.2 mV / pH，这表明一电子转移伴随着一个质子迁移。固定化的Hb对三氯乙酸（TCA）的还原表现出良好的电催化方式。