A recent eDNA-metabarcoding study assessing fish diversity in the Upper Volga catchment did not detect sterlet at any of the sampled stretches, despite recent sightings that suggest its presence. We designed a TaqMan qPCR protocol to test for sterlet in selected eDNA samples from that study. In-silico and in-vitro tests confirm the protocol’s high sensitivity and specificity to sturgeon taxa and potentially paddlefishes. Using this assay, sterlet were not detected in 26 eDNA samples from the Volga headwaters, agreeing with the metabarcoding results.

中文翻译：

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TaqMan qPCR协议的开发，用于从eDNA样品中检测伏尔加河上游水中的Acipenser ruthenus

一项最近的eDNA-metabarcoding研究评估了上伏尔加河上游流域鱼类的多样性，尽管最近发现它存在，但在任何采样段都没有检测到detect。我们设计了TaqMan qPCR方案来测试该研究中所选eDNA样品中的甾体。硅和体外测试证实了该方案对st鱼类群和潜在的pad鱼具有很高的敏感性和特异性。使用该测定法，在来自伏尔加河上游的26个eDNA样品中未检测到，与metabarcoding结果一致。