The goal of this study was to explore the inherent antioxidant and antineoplastic activities of methanolic extract of the roots of Hibiscus sabdariffa (MEHSR). The dried coarse powder of roots of Hibiscus sabdariffa was subjected to methanolic extraction. Here in vitro methods were used to determine the various types of phytochemical content and antioxidant activity of MEHSR as well as its cytotoxic effect against Ehrlich ascites carcinoma (EAC) cells. In vivo, antineoplastic activity of MEHSR against EAC cells was also evaluated by determining the viable tumor cell count, survival time, body weight gain, hematological profiles of experimental mice along with observing morphological changes of EAC cells by fluorescence microscope. Analysis of the chemical composition of MEHSR was carried out using GC-MS. Total phenolic and flavonoid contents of MEHSR were found to be 143.36 and 82.81 mg/g of extract in terms of gallic acid and catechin equivalent, respectively. The MEHSR exhibited very good scavenging property on DPPH (IC50: 13.37 μg/mL) and ABTS (IC50: 18.88 μg/mL) radicals in respect to nitric oxide (IC50: 72.82 μg/mL) radical and lipid peroxidation (IC50: 75.78 μg/mL) inhibition. MEHSR was found to induce Ehrlich ascites carcinoma (EAC) cell death at a dose dependent fashion. At dose 10 mg/kg, MEHSR significantly inhibited tumor cell growth rate (62.24%; p < 0.05), decreased tumor weight (57.81%; p < 0.05), increased life span (38.97%) compared to the untreated control mice. MEHSR also restored all hematological parameters of EAC-bearing mice towards normal level. Furthermore, administration of MEHSR induced apoptosis of EAC cells as observed in Hoechst 33342 stained cells under fluorescence microscope. Arachidic acid (49.18%), oleic acid (36.36%) and octadecanoic acid (14.47%) were identified as the major components of MEHSR by GC-MS analysis. In a nutshell, our findings proposed that MEHSR may possess promising antioxidant and antineoplastic efficacy against Ehrlich ascites carcinoma cells by induction of cell apoptosis. Therefore, it might be a potent and novel candidate for anticancer therapy.

中文翻译：

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木槿sabdariffa Linn根的抗氧化和抗肿瘤活性。对抗艾氏腹水癌细胞

这项研究的目的是探索芙蓉根（MEHSR）的甲醇提取物固有的抗氧化剂和抗肿瘤活性。将干燥的芙蓉根粗粉进行甲醇提取。在这里，体外方法用于确定MEHSR的各种植物化学成分和抗氧化活性，以及​​其对Ehrlich腹水癌（EAC）细胞的细胞毒性作用。在体内，MEHSR对EAC细胞的抗肿瘤活性也通过确定存活的肿瘤细胞数，存活时间，体重增加，实验小鼠的血液学特征以及通过荧光显微镜观察EAC细胞的形态变化来评估。使用GC-MS对MEHSR的化学组成进行分析。以没食子酸和儿茶素当量计，MEHSR的总酚和类黄酮含量分别为143.36和82.81 mg / g。相对于一氧化氮（IC50：72.82μg/ mL）和脂质过氧化（IC50：75.78μg），MEHSR对DPPH（IC50：13.37μg/ mL）和ABTS（IC50：18.88μg/ mL）自由基表现出非常好的清除性能。 / mL）抑制。发现MEHSR以剂量依赖性方式诱导艾氏腹水癌（EAC）细胞死亡。与未治疗的对照小鼠相比，MEHSR剂量为10 mg / kg时，可显着抑制肿瘤细胞的生长速度（62.24％; p <0.05），降低肿瘤重量（57.81％; p <0.05），延长寿命（38.97％）。MEHSR还使EAC荷瘤小鼠的所有血液学参数恢复到正常水平。此外，在荧光显微镜下观察到，在Hoechst 33342染色的细胞中观察到，施用MEHSR诱导EAC细胞凋亡。通过GC-MS分析，花生四烯酸（49.18％），油酸（36.36％）和十八碳烯酸（14.47％）被确定为MEHSR的主要成分。简而言之，我们的研究结果表明，MEHSR可能通过诱导细胞凋亡而具有针对Ehrlich腹水癌细胞的抗氧化剂和抗肿瘤药的功效。因此，它可能是抗癌治疗的有力和新颖的候选人。我们的研究结果表明，MEHSR可能通过诱导细胞凋亡而具有针对Ehrlich腹水癌细胞的抗氧化剂和抗肿瘤功效。因此，它可能是抗癌治疗的有力和新颖的候选人。我们的研究结果表明，MEHSR可能通过诱导细胞凋亡而具有针对Ehrlich腹水癌细胞的抗氧化剂和抗肿瘤功效。因此，它可能是抗癌治疗的有力和新颖的候选人。