In the present study, we report a reverse-phase high-performance liquid chromatography (RP-HPLC) method for separation of the regio-isomers of Glyceryl MonoRicinoleate (GMR) identified using position specificity of lipases. The approaches explored to identify these regio-isomers include LC-mass spectrometry, UV spectroscopy, and selective hydrolysis with lipases. A distinct UV absorption spectrum and λ_max values for each isomer were noted, and mass spectral analysis further revealed their molecular weight. Lastly, the purified regio-isomers were subjected to hydrolysis with two distinctive regio-specific lipases to identified as sn-2 and sn-1(3) GMR. The current methodology of using analytic tool and enzyme specificity provides a useful platform for identifying regio-isomers for structured lipid synthesis.

在本研究中，我们报告了反相高效液相色谱（RP-HPLC）方法，用于分离使用脂肪酶的位置特异性鉴定的甘油单蓖麻油酸酯（GMR）的区域异构体。鉴定这些区域异构体的探索方法包括LC质谱，UV光谱和脂肪酶选择性水解。一个明显的紫外吸收光谱和λ_最大记下每种异构体的值，质谱分析进一步揭示了它们的分子量。最后，将纯化的区域异构体用两种独特的区域特异性脂肪酶进行水解，以鉴定为sn-2和sn-1（3）GMR。使用分析工具和酶特异性的当前方法为鉴定用于结构化脂质合成的区域异构体提供了有用的平台。