This study aimed to look insights into taxonomy, evolution, and biotechnological potentials of a yeast-like fungal strain P6 isolated from a mangrove ecosystem. The genome sequencing for the yeast-like fungal strain P6 was conducted on a Hiseq sequencing platform, and the genomic characteristics and annotations were analyzed. The central metabolism and gluconate biosynthesis pathway were studied through the genome sequence data by using the GO, KOG, and KEGG databases. The secondary metabolite potentials were also evaluated. The whole genome size of the P6 strain was 25.41Mb and the G + C content of its genome was 50.69%. Totally, 6098 protein-coding genes and 264 non-coding RNA genes were predicted. The annotation results showed that the yeast-like fungal strain P6 had complete metabolic pathways of TCA cycle, EMP pathway, pentose phosphate pathway, glyoxylic acid cycle, and other central metabolic pathways. Furthermore, the inulinase activity associated with β-fructofuranosidase and high glucose oxidase activity in this strain have been demonstrated. It was found that this yeast-like fungal strain was located at root of most species of Aureobasidium spp. and at a separate cluster of all the phylogenetic trees. The P6 strain was predicted to contain three NRPS gene clusters, five type-I PKS gene clusters, and one type-I NRPS/PKS gene cluster via analysis at the antiSMASH Website. It may synthesize epichloenin A, fusaric acid, elsinochromes, and fusaridione A. Based on its unique DNA sequence, taxonomic position in the phylogenetic tree and evolutional position, the yeast-like fungal strain P6 was identified as a novel species Aureobasidium hainanensis sp. nov. P6 isolate and had highly potential applications.

中文翻译：

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酵母样真菌菌株P6（金黄色葡萄球菌的新种）的基因组测序：对其分类学，进化和生物技术潜力的见解

这项研究旨在寻找从红树林生态系统中分离出的酵母样真菌P6的分类，进化和生物技术潜力的见解。在Hiseq测序平台上对酵母样真菌菌株P6进行基因组测序，并分析了基因组特征和注释。通过使用GO，KOG和KEGG数据库通过基因组序列数据研究了中心代谢和葡萄糖酸盐的生物合成途径。还评估了次级代谢产物的潜力。P6菌株的整个基因组大小为25.41Mb，其基因组的G + C含量为50.69％。总共预测出6098个蛋白质编码基因和264个非编码RNA基因。注释结果表明，酵母样真菌菌株P6具有完整的TCA循环代谢途径，EMP途径，磷酸戊糖途径，乙醛酸循环和其他中枢代谢途径。此外，已经证明了该菌株中与β-果糖呋喃糖苷酶相关的菊粉酶活性和高葡萄糖氧化酶活性。发现该酵母样真菌菌株位于大多数金黄色葡萄球菌属物种的根部。并在所有系统发育树的单独群集中。通过在antiSMASH网站上进行的分析，预测P6菌株包含三个NRPS基因簇，五个I型PKS基因簇和一个I型NRPS / PKS基因簇。它可以合成上皮蛋白A，岩藻酸，elsinochromes和fusa​​ridioneA。基于其独特的DNA序列，系统树中的分类位置和进化位置，酵母样真菌菌株P6被鉴定为海南金黄色葡萄球菌的新种。十一月