Salt stress is first perceived by the plant roots and inhibits plant growth in the short-term by inducing osmotic stress caused by decreased water availability. In this study, 160 mM NaCl was applied to three-leaf-stage barley plants (Hordeum vulgare L. cv. Martı) for a short period (0, 2, and 26 h) Osmolyte accumulation and ion leakage was significantly increased after salt stress treatment compared with control conditons in both leaf and root tissues within 2 h. We have also found that expressions of transcription factors HvDRF2 and HvWRKY12, associated with abiotic stress including salinity and drought stress, were quite low in root and shoots in control conditions; however, salt stress resulted into upregulation of HvDRF2 expression as 28.8- and 26.6-fold in roots and leaves, respectively, within 26 h. While salt stress-induced significantly upregulation of HvPR1A (26.4-fold) HvNHX1 (sevenfold) in 2 h at P < 0.05 level, significant upregulation of HvMT2 (8.2-fold) and HvDHN3 (4.7-fold) was observed at 26 h after salt treatment in roots. In leaves, HvMT2 (12.7-fold), HvNHX1 (12.1-fold) and HvBAS1 (3.4-fold) were significantly upregulated under salt stress. Only HvLHCB mRNA level was significantly decreased as 2- and 5.6-fold in leaf tissues with salinityin 2 and 26 h, respectively.

中文翻译：

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盐胁迫下数小时内三叶期大麦（Hordeum vulgare L.）的差异生理和分子响应

盐胁迫首先被植物的根部感知到，并在短期内通过诱导因水分利用率降低而引起的渗透胁迫而抑制植物的生长。在这项研究中，将160 mM NaCl短时间（0、2和26 h）施用于三叶大麦植物（大麦（Hordeum vulgare L. cv。Martı）），渗透盐的积累和离子泄漏在盐胁迫后显着增加在2小时内，在叶片和根部组织中，与对照相比，这种处理方法的效果更好。我们还发现，转录因子表达HvDRF2和HvWRKY12，非生物胁迫，包括盐碱和干旱胁迫相关的，是在根和在控制条件下芽相当低; 但是，盐胁迫会导致盐分上调HvDRF2表达28.8-和在根和叶分别26.6倍，26小时之内。在P  <0.05水平下，盐胁迫在2 h内引起HvPR1A（26.4倍）HvNHX1（7倍）显着上调，而在盐后26 h观察到HvMT2（8.2倍）和HvDHN3（4.7倍）显着上调。根治。在盐胁迫下，叶片中的HvMT2（12.7倍），HvNHX1（12.1倍）和HvBAS1（3.4倍）显着上调。在含盐度为2和26 h的叶片组织中，只有HvLHCB mRNA水平显着降低了2倍和5.6倍。