The Arabidopsis thaliana genome contains many genes that encode proteins with a domain of unknown function (DUF). To improve our understanding of the functions of these DUF family members, a DUF761-containing gene at the At4g16790 locus was isolated and designated DUF761-1. Duf761-1 loss-of-function mutant did not significantly affect plant growth and development, whereas transgenic plants overexpressing DUF761-1 exhibited several unique phenotypes during vegetative and reproductive growth. DUF761-1 overexpression was associated with altered leaf and root morphology, inhibition of inflorescence stem elongation and thickening, defects in flower development, and reduced seed production compared to that of wild-type plants. Moreover, DUF761-1 overexpressing plants exhibited constitutively activated plant defense responses and displayed enhanced resistance to Pseudomonas syringae L. pv. tomato DC3000 during pathogen infection assays. Taken together, the combination of characterization of plant phenotypes, gene expression analysis and pathogen resistance assays in loss-of-function mutant and overexpression lines suggests that DUF761-1 may be involved in plant development and defense responses.

中文翻译：

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拟南芥DUF761含有蛋白的表征，在发育和防御反应中具有潜在作用

在拟南芥基因组含有许多基因与未知功能（DUF）的结构域编码的蛋白质。为了提高我们的这些DUF家族成员的功能的理解，在At4g16790基因含有DUF761基因分离出来，并指定DUF761 - 1。Duf761 - 1丧失功能的突变并未显著影响植物的生长和发育，而转基因植物中过表达DUF761 - 1个营养生长和生殖生长过程中表现出一些独特的表型。DUF761 - 1与野生型植物相比，过表达与叶和根形态的改变，花序茎的伸长和增厚的抑制，花发育的缺陷以及种子产量的减少有关。此外，DUF761 - 1种过表达植物显示组成性活化的植物防御反应并显示增强的抗性丁香假单胞菌L. PV。番茄DC3000在病原体感染测定中。两者合计，植物表型，基因表达分析和病原抗性测定的表征在失功能突变体和过表达株系的组合表明，DUF761 - 1 可能参与植物发育和防御反应。