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Cytochrome P450s in the sugarcane Saccharum spontaneum
Tropical Plant Biology ( IF 1.8 ) Pub Date : 2019-04-24 , DOI: 10.1007/s12042-019-09226-2
David R. Nelson

The Cytochrome P450 gene family is one of the largest in plants. Cytochrome P450 sequences have been annotated in the Saccharum spontaneum genome and they make up 1.3% of the known alleles. 394 genes have been identified in this family after mining two releases of the S. spontaneum AP85–441 genome. The P450 sequences are most similar to those of sorghum. The 45 CYP families in sugarcane have been compared to sorghum P450s. CYP51 was the most similar sequence at 98.6%. Average percent identity to the best plant blast match was 86%. Three of the 45 CYP families show modest gene expansion. CYP71 has 15 more genes CYP76 has 12 more genes and CYP81 has 6 more genes than sorghum. The other families are identical or within 4 genes, showing a strong similarity between P450s in the two species. Alleles have been assigned for this tetraploid genome. Only 32% of the genes still have four alleles.

中文翻译:

甘蔗糖浆中的细胞色素P450

细胞色素P450基因家族是植物中最大的家族之一。细胞色素P450序列已在自然酿酒酵母基因组中注释,它们占已知等位基因的1.3%。在挖掘两个版本的自发链球菌后,已鉴定出394个基因AP85–441基因组。P450序列与高粱序列最相似。将甘蔗中的45个CYP家族与高粱P450进行了比较。CYP51是最相似的序列,为98.6%。最佳植物爆炸匹配的平均同一性百分比为86%。45个CYP家族中有3个显示适度的基因扩增。CYP71比高粱多15个基因CYP76比高粱多12个基因,CYP81比高粱多6个基因。其他家族相同或在4个基因内,显示两个物种中的P450之间有很强的相似性。已经为该四倍体基因组分配了等位基因。仅32%的基因仍具有四个等位基因。
更新日期:2019-04-24
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