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Nuclear and plastid SNP markers for tracing Cedrela timber in the tropics
Conservation Genetics Resources ( IF 0.9 ) Pub Date : 2019-08-09 , DOI: 10.1007/s12686-019-01110-1
Kathelyn Paredes-Villanueva , Celine Blanc-Jolivet , Malte Mader , Eurídice N. Honorio Coronado , Carmen Garcia-Davila , Alexandre M. Sebbenn , Barbara Rocha Venancio Meyer-Sand , Henri Caron , Niklas Tysklind , Stephen Cavers , Bernd Degen

Illegal logging and trade of timber are major worldwide concerns, resulting in biodiversity and economic losses. Tropical tree species in the genus Cedrela, which have historically been heavily exploited, are still often illegally traded and there is an urgent need to develop tools to verify the origin of Cedrela products. A set of 351 SNP loci for Cedrela species from Bolivia, Brazil, Costa Rica, Cuba, Ecuador, French Guiana, Mexico, and Peru was developed using restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing, and adapted for MassARRAY genotyping. After screening of 94 individuals covering most of the distribution of Cedrela, a final set of 136 SNP loci which included 92 nuclear SNPs, 22 chloroplast markers (20 SNPs and 2 INDELs), and 22 mitochondrial markers (19 SNPs and 3 INDELs) was selected and tested for potential to verify Cedrela timber origin.



中文翻译:

核和质体SNP标记,用于在热带地区追踪Cedrela木材

木材的非法采伐和贸易是世界范围内的主要问题,导致生物多样性和经济损失。塞德拉(Cedrela)属的热带树种在历史上已被大量开发,但仍经常非法交易,迫切需要开发工具来验证塞德拉(Cedrela)产品的来源。使用限制相关DNA测序(RADSeq)和低覆盖率MiSeq基因组测序开发了一组来自玻利维亚,巴西,哥斯达黎加,古巴,厄瓜多尔,法属圭亚那,墨西哥和秘鲁的Cedrela物种的351个SNP位点,并适用于MassARRAY基因分型。在筛选了涵盖塞德雷拉大部分分布的94个人之后,最终选择了136个SNP位点,其中包括92个核SNP,22个叶绿体标记(20个SNP和2个INDEL)和22个线粒体标记(19个SNP和3个INDEL),并测试了验证Cedrela木材起源的潜力。

更新日期:2019-08-09
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