Peptones are accepted as one of the most expensive medium components of microorganisms. The present study was undertaken to investigate the effect of chicken feather peptone (CFP) on enzyme (lipase and amylase) production by Bacillus licheniformis 016. In order to assess its effectiveness on enzyme production, CFP was compared with commercial fish peptone (FP) and protease peptone (PP). The optimum concentration of CFP for lipase and amylase production was determined as 5 and 6 g/L, respectively. The optimum concentration of both FP and PP was found as 4 g/L for lipase production and 5 g/L for amylase production. In all the peptone media, the optimal incubation times for amylase and lipase production were determined as 24 and 48 h, respectively. CFP was found to be more favorable for lipase and amylase production. In CFP, PP and FP media, the maximum lipase activities were 1870, 1582 and 1831 U/L, and the maximum amylase activities were 1680, 1505 and 632 U/L, respectively. On the other hand, better cell growth performance was achieved in CFP media compared to PP and FP media. The least pH change was detected in CFP-containing media. CFP was also found to prevent starch aggregation in the medium in contrast to FP and PP. This study exhibited that CFP was a better nitrogen source or an inducer for lipase and amylase production as well as cell growth in comparison to the tested commercial peptones.

中文翻译：

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利用废弃鸡羽毛作为蛋白ept来源增强地衣芽孢杆菌016淀粉酶和脂肪酶的生产

蛋白ept被认为是微生物中最昂贵的培养基成分之一。本研究旨在调查鸡羽毛蛋白one（CFP）对地衣芽孢杆菌产生酶（脂肪酶和淀粉酶）的影响016.为了评估其对酶产生的有效性，将CFP与商品鱼蛋白ept（FP）和蛋白酶蛋白ept（PP）进行了比较。确定用于脂肪酶和淀粉酶的CFP的最佳浓度分别为5和6g / L。FP和PP的最佳浓度分别为：脂肪酶生产4 g / L和淀粉酶生产5 g / L。在所有蛋白ept培养基中，淀粉酶和脂肪酶生产的最佳孵育时间分别确定为24 h和48 h。发现CFP对脂肪酶和淀粉酶的生产更有利。在CFP，PP和FP介质中，最大脂肪酶活性分别为1870、1582和1831 U / L，最大淀粉酶活性分别为1680、1505和632 U / L。另一方面，与PP和FP培养基相比，在CFP培养基中获得了更好的细胞生长性能。在含CFP的培养基中检测到的pH值变化最小。与FP和PP相比，还发现CFP可以防止培养基中的淀粉聚集。这项研究表明，与测试的商业蛋白ept相比，CFP是更好的氮源或脂肪酶和淀粉酶产生以及细胞生长的诱导剂。