Backgrounds

Exposure to a toxic stress environment leads to excessive inflammatory reactions and induces allergic asthma resulting in airway hyper-responsiveness. We investigated whether Rosa laevigata Michx. (RL) exhibits anti-inflammatory effects related allergic asthma in both an in vitro and in vivo studies.

Methods

To investigate the preventive effect of RL, A549 cells were pretreated with non-toxic doses of RL (500, 1000 μg/mL) and induced by epidermal growth factor (EGF) (10 ng/mL). First, we evaluated cytotoxicity via a MTT assay. The inhibitory effects of NF-κB activity and COX-2 expression were confirmed by a western blot assay. In the in vivo study, BALB/c mice were challenged with regard to ovalbumin via an intraperitoneal injection of RL (50, 100 mg/kg) and were killed to collect bronchoalveolar lavage fluid, lung tissues and blood serum. The number of inflammatory cells, the secretion of IgE and related cytokines were monitored by ELISA and multiplex assays.

Results

RL significantly suppressed NF-κB activity and COX-2 expression levels in EGF-induced A549 cells. In a chronic inflammation mice model, pretreatment of RL attenuated allergic airway inflammation by reducing inflammatory cells, the secretion of IgE and related cytokines in a dose-dependent manner.

Conclusions

The results of this study present the possibility of RL as a therapeutic agent for allergic asthma patients via the suppression of IgE and related cytokines.

中文翻译：

---

罗莎叶提取物通过抑制IgE及相关细胞因子对过敏性哮喘体内外模型的抗炎作用

背景资料

暴露于有毒的压力环境中会导致过度的炎症反应，并引起过敏性哮喘，导致呼吸道反应过度。我们调查了Rosa laevigata Michx。（RL）在体外和体内研究中均显示出与过敏性哮喘相关的抗炎作用。

方法

为了研究RL的预防作用，将A549细胞用无毒剂量的RL（500，1000μg/ mL）预处理，并通过表皮生长因子（EGF）（10 ng / mL）诱导。首先，我们通过MTT分析评估了细胞毒性。蛋白质印迹法证实了NF-κB活性和COX-2表达的抑制作用。在体内研究中，通过腹膜内注射RL（50，100 mg / kg）对BALB / c小鼠的卵清蛋白进行攻击，并杀死其以收集支气管肺泡灌洗液，肺组织和血清。通过ELISA和多重测定法监测炎症细胞的数目，IgE的分泌和相关细胞因子。

结果

RL显着抑制EGF诱导的A549细胞中NF-κB活性和COX-2表达水平。在慢性炎症小鼠模型中，RL的预处理通过减少炎症细胞，IgE和相关细胞因子的分泌以剂量依赖的方式减轻了过敏性气道炎症。

结论

这项研究的结果表明，通过抑制IgE和相关细胞因子，RL可作为过敏性哮喘患者的治疗剂。