In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections between endothelial capillaries of heterogeneous sections (5–100 μm in diameter) and artificial plastic tubes or reservoirs (1–10 mm in diameter). Moreover, the micro-VB device can be installed in a standard 100 mm-diameter Petri dish. Endothelial networks in 3D engineered tissues were obtained by cellular self-assembly on the device, after co-culturing of human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) in fibrin gel. Endothelial capillary connection between vascularized tissues and microfluidic channels, mimicking arteries and veins, was confirmed by perfusion of fluorescent microspheres. The micro-VB devices were compatible with the use of commercially available culture dishes and did not require the involvement of additional equipment. Thus, these micro-VB devices are expected to substantially improve the routine application of 3D tissue engineering to regenerative medicine.

中文翻译：

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用于血管化3D组织工程的微流体血管床设备：芯片上的组织工程。

在此报告中，我们描述了一种微流体血管床（micro-VB）设备，该设备为具有血管网络形成的3D组织工程提供了平台。微型VB设备允许异质部分的内皮毛细管（直径为5-100μm）与人造塑料管或容器（直径为1-10 mm）之间进行功能连接。此外，可以将微型VB设备安装在直径为100毫米的标准培养皿中。在纤维蛋白凝胶中共同培养人脐静脉内皮细胞（HUVEC）和正常人真皮成纤维细胞（NHDF）后，通过设备上的细胞自组装获得3D工程组织中的内皮网络。通过荧光微球的灌注证实了血管化组织和微流体通道之间的内皮毛细管连接，模拟了动脉和静脉。微型VB设备可与市售培养皿兼容使用，不需要其他设备。因此，这些微型VB设备有望大大改善3D组织工程在再生医学中的常规应用。