Abstract

Quantifying rotavirus shedding among vaccinated individuals will aid understanding of vaccine indirect effects. Serial stool samples were collected from 196 children who presented with rotavirus gastroenteritis to health facilities in Blantyre, Malawi, and were tested for rotavirus using a VP6 semi-quantitative, real-time polymerase chain reaction. The median duration of fecal shedding was 28 days (95% CI, 19–28). The median copy numbers for peak shedding were 1.99 × 10⁷ (interquartile range, 3.39 × 10⁶ to 6.37 × 10⁷). The fecal viral load was positively associated with disease severity and negatively associated with serum anti-rotavirus immunoglobin A. High and persistent rotavirus shedding among vaccinated children with breakthrough disease may contribute to ongoing transmission in this setting.

中文翻译：

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接种轮状病毒性胃肠炎的马拉维儿童中粪便轮状病毒脱落的持续时间和密度

摘要

定量接种疫苗者中轮状病毒的脱落将有助于了解疫苗的间接作用。从196名患轮状病毒肠胃炎的儿童的大便样本中收集样本，这些儿童到马拉维的布兰太尔的医疗机构，并使用VP6半定量实时聚合酶链反应检测轮状病毒。粪便脱落的中位时间为28天（95％CI，19-28）。峰值脱落的平均拷贝数为1.99×10 ⁷（四分位间距为3.39×10 ⁶至6.37×10 ⁷）。粪便病毒载量与疾病严重程度呈正相关，与血清抗轮状病毒免疫球蛋白A呈负相关。在具有突破性疾病的接种疫苗的儿童中，轮状病毒的高而持续性脱落可能有助于这种情况下的持续传播。