Nuclear morphology studies are a reliable parameter for analysis of genotoxic properties of different agents. Such studies have been widely exploited in isolated protoplasts but seldom used in cells isolated from whole root tissue due to cell wall permeability restrictions. We present a rapid and easy protocol that will benefit the researchers in studying the nucleus stained with fluorescent dye. Here, we have modified the digestion buffer and used a permeabilization buffer to ease the entry of the fluorescent Hoechst stain through the cell wall, so that fluorescence can be observed under UV light.

中文翻译：

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从水稻全根组织中Hoechst（33258）染色核的基本方法

核形态学研究是分析不同药物遗传毒性的可靠参数。此类研究已广泛用于分离的原生质体中，但由于细胞壁通透性的限制，很少用于从整个根组织分离的细胞中。我们提出了一种快速，简便的方案，对研究荧光染料染色的细胞核将有利于研究人员。在这里，我们修改了消化缓冲液，并使用了透化缓冲液，以减轻荧光赫斯特染料通过细胞壁的进入，从而可以在紫外光下观察荧光。