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Methods for the determination of glucose in blood. Part 1
Reviews and Advances in Chemistry Pub Date : 2015-02-12 , DOI: 10.1134/s207997801501001x
V. A. Buzanovskii

The chronological development of destructive and nondestructive methods designed to determine the glucose concentration in plasma, serum, and whole blood is presented in the review. Destructive procedures are divided into spectrophotometric, spectrofluorimetric, diffraction, amperometric, potentiometric, conductometric, chromatographic, gas chromatographic-mass spectrometric, titrimetric, and calorimetric, as well as procedures of reflectance photometry. A brief overview of these methods and the results of comparative tests are given; the procedures are systematized. It is noted that certain types of enzymatic spectrophotometric and amperometric methods have gained wide acceptance for analyses in clinical diagnostic laboratories. Outside clinical laboratories, enzymatic amperometric and spectrophotometric procedures and reflectance photometry procedures using test strips are widely used. Chromatography-mass spectrometry procedures are often applied to certify standard serum samples. Spectrofluorimetric, diffraction, and chromatographic techniques are common in scientific biomedical research. Nondestructive methods like nonenzymatic spectrophotometric, nonenzymatic amperometric, potentiometric, conductometric, titrimetric, and calorimetric procedures, as well as a number of enzymatic spectrophotometric procedures, in most cases, are not used in modern clinical and diagnostic practice.

中文翻译:

测定血液中葡萄糖的方法。第1部分

评论介绍了旨在确定血浆,血清和全血中葡萄糖浓度的破坏性和非破坏性方法的时间顺序发展。破坏性程序分为分光光度法,分光荧光法,衍射法,电流法,电位法,电导法,色谱法,气相色谱-质谱法,滴定法和量热法,以及反射光度法。简要概述了这些方法以及比较测试的结果;程序是系统的。值得注意的是,某些类型的酶法分光光度法和安培法已被临床诊断实验室的分析广泛接受。外部临床实验室 使用试纸的酶安培和分光光度法程序以及反射光度法程序被广泛使用。色谱-质谱法通常用于鉴定标准血清样品。荧光光谱,衍射和色谱技术在科学的生物医学研究中很常见。在大多数情况下,现代临床和诊断实践中未使用非破坏性方法,例如非酶法,光度法,电位法,电导法,滴定法和量热法,以及许多酶法。和色谱技术在生物医学科学研究中很常见。在大多数情况下,现代临床和诊断实践中未使用非破坏性方法,例如非酶法,光度法,电位法,电导法,滴定法和量热法,以及许多酶法。和色谱技术在生物医学科学研究中很常见。在大多数情况下,现代临床和诊断实践中未使用非破坏性方法,例如非酶法,光度法,电位法,电导法,滴定法和量热法,以及许多酶法。
更新日期:2015-02-12
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