The aim of this study was to evaluate the effect of riboflavin supplementation of culture medium on conidial UV-A tolerance of M. acridum (Driver & Milner) (Hypocreales: Clavicipitaceae) and M. robertsii (Bischoff, Rehner & Humber) (Hypocreales: Clavicipitaceae). These fungi were produced on culture medium supplemented, or not supplemented, with riboflavin. Relative germination and expression patterns of some photoprotection-related genes were evaluated after irradiating with artificial UV-A, or with filtered solar radiation (> 320 nm; UV-A and visible radiation). M. acridum conidia harvested from riboflavin-supplemented culture medium demonstrated enhanced UV-A tolerance when irradiated with artificial UV-A. Nevertheless, relative germination of conidia of both species produced on riboflavin-supplemented medium and exposed to filtered solar radiation was significantly higher than those produced on medium not supplemented with riboflavin. Riboflavin increased the transcription of photolyases, laccases and polyketide synthase genes. However, each fungal species induced different genes patterns involved in DNA repair and photoprotection. The addition of riboflavin to the substrate used for mass production of Metarhizium spp. and the resulting enhancement of conidial tolerance to solar radiation may improve the effectiveness of these fungi in biological control programs.

中文翻译：

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在补充了核黄素的培养基上产生的罗伯氏梅氏菌和a霉分生孢子具有增加的UV-A耐受性并上调了光保护和光活化基因

这项研究的目的是评估培养基中核黄素的添加对cri霉（Driver＆Milner）（Hypocreales：Clavicipitaceae）和robertsii（Bischoff，Rehner＆Humber）（Hypocreales：锁骨科）。这些真菌在补充或不补充核黄素的培养基上产生。在用人工UV-A或过滤的太阳辐射（> 320 nm； UV-A和可见光）照射后，评估了一些与光保护相关基因的相对萌发和表达模式。cri从补充了核黄素的培养基中收获的分生孢子经人工UV-A照射后，对UV-A的耐受性增强。然而，在补充了核黄素的培养基上并暴露于过滤的太阳辐射下，这两种物种分生孢子的相对发芽率明显高于未补充核黄素的培养基上产生的分生孢子。核黄素增加了光解酶，漆酶和聚酮化合物合酶基因的转录。但是，每种真菌物种诱导涉及DNA修复和光保护的不同基因模式。核黄素的添加到衬底用于大量生产的绿僵spp。最终提高了分生孢子对太阳辐射的耐受性，从而可以提高这些真菌在生物防治程序中的有效性。