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Krüppel homologue 1 interacts directly with Hairy and regulates ecdysis in the brown planthopper.
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2020-01-21 , DOI: 10.1111/imb.12635 Y Mao 1 , Y Li 1 , H Gao 1 , X Lin 1
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2020-01-21 , DOI: 10.1111/imb.12635 Y Mao 1 , Y Li 1 , H Gao 1 , X Lin 1
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Juvenile hormone (JH) plays important roles in the growth and development of insects. JH and its receptor methoprene‐tolerant (Met) regulate the expression of transcription factors to control the transcription of downstream genes. The expression of Hairy (Hry) and Krüppel homologue 1 (Kr‐h1) is regulated by JH and JH receptors. Hry and Kr‐h1 are both crucial in mediating JH signalling. However, whether they interact at the gene level in regulating metamorphosis and whether they interact physically at the protein level remain unknown. We used co‐immunoprecipitation, glutathione S‐transferase pull‐down and RNA interference (RNAi) approaches to study the genetic and biochemical interactions of the two proteins Hry and Kr‐h1. The results showed that brown planthopper (Nilaparvata lugens) Hry and Kr‐h1 interact directly: Hry binds to the N‐terminal of Kr‐h1, which includes five zinc‐finger domains. The RNAi experiment showed that downregulation of Hry reduced the ratio of ecdysis failure caused by knockdown of Kr‐h1, indicating that the downregulation of Hry might mitigate ecdysis failure via the downregulation of Kr‐h1. The expression of Hry increased significantly when Kr‐h1 was downregulated, whereas it did not change significantly when both were downregulated. Our results suggest that the binding of Hry protein with Kr‐h1 prevents the N‐terminal five zinc‐finger domains from binding with DNA, which in turn inactivates the transcription activator or inhibitor function of Kr‐h1. Hry could possibly be used as a target for pesticide applications in the future.
中文翻译：
Krüppel同系物1与Hairy直接相互作用，并调节褐飞虱的蜕皮。
少年激素（JH）在昆虫的生长发育中起着重要作用。JH及其受体甲氨蝶呤耐受（Met）调节转录因子的表达，以控制下游基因的转录。的表达毛状（HRY）和Krüppel同源物1（氪-H1）由JH和JH受体的调节。Hry和Kr-h1在调解JH信号方面都至关重要。然而，它们是否在调节变态的基因水平上相互作用以及它们是否在蛋白质水平上物理相互作用仍然未知。我们使用了共免疫沉淀，谷胱甘肽S-转移酶下拉和RNA干扰（RNAi）方法来研究两种蛋白Hry和Kr-h1的遗传和生化相互作用。结果表明，褐飞虱Hry和Kr-h1直接相互作用：Hry与Kr-h1的N末端结合，后者包括5个锌指结构域。RNAi实验表明，Hry的下调降低了由敲除Kr-h1引起的蜕皮失败的比率，这表明Hry的下调可能通过下调Kr-h1减轻蜕皮失败。下调Kr-h1时，Hry的表达显着增加，而当下调Kr-h1时，Hry的表达没有明显改变。我们的结果表明，Hry蛋白与Kr-h1的结合可阻止N末端的五个锌指结构域与DNA结合，从而使Kr-h1的转录激活因子或抑制剂功能失活。未来，Hry可能会成为农药应用的目标。
更新日期：2020-01-21
中文翻译：
Krüppel同系物1与Hairy直接相互作用，并调节褐飞虱的蜕皮。
少年激素（JH）在昆虫的生长发育中起着重要作用。JH及其受体甲氨蝶呤耐受（Met）调节转录因子的表达，以控制下游基因的转录。的表达毛状（HRY）和Krüppel同源物1（氪-H1）由JH和JH受体的调节。Hry和Kr-h1在调解JH信号方面都至关重要。然而，它们是否在调节变态的基因水平上相互作用以及它们是否在蛋白质水平上物理相互作用仍然未知。我们使用了共免疫沉淀，谷胱甘肽S-转移酶下拉和RNA干扰（RNAi）方法来研究两种蛋白Hry和Kr-h1的遗传和生化相互作用。结果表明，褐飞虱Hry和Kr-h1直接相互作用：Hry与Kr-h1的N末端结合，后者包括5个锌指结构域。RNAi实验表明，Hry的下调降低了由敲除Kr-h1引起的蜕皮失败的比率，这表明Hry的下调可能通过下调Kr-h1减轻蜕皮失败。下调Kr-h1时，Hry的表达显着增加，而当下调Kr-h1时，Hry的表达没有明显改变。我们的结果表明，Hry蛋白与Kr-h1的结合可阻止N末端的五个锌指结构域与DNA结合，从而使Kr-h1的转录激活因子或抑制剂功能失活。未来，Hry可能会成为农药应用的目标。
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