Abstract

Wolbachia are intracellular symbiotic bacteria of terrestrial arthropods and some nematodes. They are found in most insect orders; however, there are insufficient data on symbiont distribution patterns for some taxonomic groups. Here, we examined a collection of Siphonaptera species by conventional and nested polymerase chain reaction. A total of 722 specimens from 30 species were sampled in three regions of Russia: Southern Russia, Ural and the Far East. Wolbachia infection was found in half of the species, which confirmed previous data on the widespread nature of the infection in Siphonaptera. No statistical differences in Wolbachia incidence in flea species from Southern Russia and the Far East were detected, although species lists of these regions completely differed. We did not find totally infected flea species, although high infection rates were detected for Frontopsyllaelatabotis (64.5%) and Ctenophthalmuswagneri (66%) with sample size exceeding 30 specimens. Our results are in agreement with the data from other regions of the world. Combined data of our study and other authors indicate that Wolbachia symbionts are found in all 11 studied families of Siphonaptera and in 45 out of about 120 studied species in the world.

中文翻译：

---

跳蚤种群中的Wolbachia感染（昆虫：Siphonaptera）

摘要

Wolbachia是陆生节肢动物和某些线虫的细胞内共生细菌。在大多数昆虫订单中都可以找到它们。但是，对于某些生物分类群，共生体分布模式的数据不足。在这里，我们通过常规和嵌套式聚合酶链反应检查了虹吸虫物种的集合。在俄罗斯三个地区（俄罗斯南部，乌拉尔和远东）共采样了来自30个物种的722个标本。在该物种的一半中发现了Wolbachia感染，这证实了以前有关虹吸虫感染的广泛性的数据。Wolbachia无统​​计学差异尽管发现了俄罗斯南部和远东地区的跳蚤物种，但这些物种的物种清单完全不同。我们没有发现完全感染跳蚤的物种，尽管检出的Frontopsylla elata botis（64.5％）和Ctenophthalmus wagneri（66％）的检出率很高，样本数量超过30个。我们的结果与世界其他地区的数据一致。我们的研究和其他作者的综合数据表明，沃尔巴克氏菌共生体在所有11个被研究的虹吸虫科中以及世界上大约120个被研究物种中的45个中被发现。