A versatile and effective method of producing polyvinyl alcohol (PVA)-xylanase (XY) fibers cross-linked by glutaraldehyde vapor (GA) is reported in the present study. Crosslinking was performed for 30, 60, 90, and 180 min. The morphology, structure, and thermal stability of the fibers were investigated by scanning electron microscopy (SEM), Fourier-transformed infrared spectroscopy (FTIR), and thermogravimetric analyses (TGA). The enzymatic activity was studied under different storage time and reutilization cycles. The enzyme’s ability to reduce the turbidity of a standard β–glucan solution was also verified. Cross-linked fibers presented denser and more compacted structures. There was an improvement in thermal properties as well as in the recycling and storage efficiency of cross-linked XY–PVA fibers, compared to their non-cross-linked counterparts. Free and 180 min-cross-linked immobilized enzymes were able to reduce the turbidity of the β-glucan solution by a similar degree.

中文翻译：

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戊二醛交联的电纺聚乙烯醇-木聚糖酶纤维的催化效率，结构和再循环行为

在本研究中报告了一种通用且有效的方法来生产通过戊二醛蒸气（GA）交联的聚乙烯醇（PVA）-木聚糖酶（XY）纤维。交联进行30、60、90和180分钟。通过扫描电子显微镜（SEM），傅立叶变换红外光谱（FTIR）和热重分析（TGA）研究了纤维的形态，结构和热稳定性。在不同的储存时间和再利用周期下研究了酶活性。还证实了该酶降低标准β-葡聚糖溶液浊度的能力。交联纤维呈现出更致密和更致密的结构。交联的XY-PVA纤维的热性能以及回收利用和储存效率都有所提高，与非交联的同类相比。游离和180分钟交联的固定化酶能够以相似的程度降低β-葡聚糖溶液的浊度。