The aim of this study was to identify a Mexican native basidiomycete strain with antagonistic potential against phytopathogenic fungi. The antifungal activity of fifteen isolates against Fusarium oxysporum, Fusarium solani, Colletotrichum truncatum, Colletotrichum coccodes, Colletotrichum sp., Stemphylium solani, Botrytis cinerea, Epicoccum nigrum, Phomopsis sp. and Alternaria solani was studied in vitro using dual culture assay. An isolate of Macrolepiota sp (CS185) exhibited the strongest ability to compete on potato dextrose agar medium (PDA) causing 22.2 up to 46.6% inhibition in seven out of ten pathogens evaluated. The addition of 30% Macrolepiota sp. liquid filtrates (from 7 day-cultures in potato dextrose broth) to PDA plates inhibited 66% of A. solani growth. Application of Macrolepiota sp. CS185 culture fluids to control early blight disease caused in tomato plants by A. solani under greenhouse conditions diminished disease incidence and percent of damage. The length of the stems, the roots and the foliar area increased in the treated plants and, above all, the foliar symptoms decreased by 73%. Sesquiterpene lactones and quinones identified in culture fluids might be responsible for Macrolepiota antifungal activity. This study proves that Macrolepiota sp. CS185 culture filtrates have potential in biocontrol of early blight disease.

中文翻译：

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Macrolepiota sp。的拮抗潜力。对抗番茄疫病病原体的病原体-茄念珠菌

这项研究的目的是确定墨西哥本地担子菌菌株具有对抗植物病原真菌的潜力。十五种分离物对尖孢镰刀菌，茄镰刀菌，圆头炭疽菌，炭疽菌，coletotrichum sp。，Stemphylium solani，灰葡萄孢，灰葡萄球菌，黑附球菌，Phomopsis sp。的抗真菌活性。和番茄早疫病菌，研究在体外使用双培养试验。Macrolepiota sp（CS185）的分离物表现出最强的与马铃薯葡萄糖琼脂培养基（PDA）竞争的能力，在所评估的十种病原体中有七种引起22.2到46.6％的抑制。添加30％Macrolepiotasp。液体滤液（在马铃薯葡萄糖肉汤中培养7天）至PDA平板抑制了sol。A. solani的生长。Macrolepiota sp。的应用 CS185培养液可控制温室条件下番茄A. solani引起的早疫病，从而减少了发病率和危害百分率。处理过的植物的茎长，根长和叶面面积增加，最重要的是，叶面症状减少了73％。在培养液中鉴定出的倍半萜内酯和醌可能是Macrolepiota抗真菌活性的原因。这项研究证明Macrolepiota sp。CS185培养滤液在早期疫病的生物防治中具有潜力。