Pitaya (Hylocereus polyrhizus) is a highly valued functional food that is widely planted in Southeast Asia. However, with increased soil salinization in cultivation areas, a better understanding of the molecular mechanisms associated with salt stress responses in red pitaya is necessary. Herein, RNA-Seq was used to de novo assemble and characterize the transcriptomic profiles of red pitaya roots in response to salt stress. A total of 73,589 transcripts were obtained and the average sequence length was 1308 bp. From these transcripts, 26,878 unique transcripts were successfully matched to 13,519 Swiss-prot proteins. Gene ontology (GO) annotations showed that within the categories of molecular function, biological function, and cell component, catalytic activity (GO:0003824), metabolic process (GO:0008152), and cell part (GO:0044464) were the most enriched, respectively. 2624 transcripts were significantly differentially expressed among three time points (3 h, 7 h, and 30 h) following exposure to 450 mM NaCl. Furthermore, 261 genes were up-regulated and 61 down-regulated in all three of the time points. Glycolysis/gluconeogenesis was one of the most significantly modulated pathways. The findings presented herein provide further insight into salt stress responses in pitaya and will provide a valuable resource for future functional studies examining salt adaptations.

中文翻译：

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火龙果根的RNA序列从头组装和对盐胁迫的差异转录组分析。

火龙果（Hylocereus polyrhizus）是一种在东南亚广泛种植的极有价值的功能食品。然而，随着耕地土壤盐碱化程度的提高，有必要更好地了解与红火龙果中盐分胁迫反应相关的分子机制。在本文中，RNA-Seq用于从头组装和表征响应盐胁迫的红色火龙果根的转录组谱。总共获得了73,589个转录物，平均序列长度为1308 bp。从这些转录本中，成功地将26,878个独特的转录本与13,519个Swiss-prot蛋白进行了匹配。基因本体论（GO）注释显示，在分子功能，生物学功能和细胞成分的类别中，催化活性（GO：0003824），代谢过程（GO：0008152）和细胞部分（GO：0044464）含量最高， 分别。暴露于450 mM NaCl后的三个时间点（3小时，7小时和30小时）中2624个转录物显着差异表达。此外，在所有三个时间点中，有261个基因上调，有61个基因下调。糖酵解/糖异生是最显着的调节途径之一。本文介绍的发现提供了对火龙果中盐胁迫响应的进一步了解，并将为将来的研究盐适应性功能研究提供宝贵的资源。