ABSTRACT Macroalgae of the genera Fucus, Ulva, and Enteromorpha are typically abundant in estuaries. Endophytic fungi may have beneficial effects on the hosts affecting their ability to cope with stress. They are also a source of biologically active compounds. However, little is known about the endophytic fungi that colonize these macroalgae. Endophytic isolates were obtained from macroalgae from various sites in the estuary Ria de Aveiro (Portugal), as well as from saline water and sponges. Six Acremonium-like species could not be affiliated to any known species. Phylogenetic analyses based on internal transcribed spacer (ITS) region of the ribosomal DNA and β-tubulin (tub2) and actin (act1) genes placed these species in the genera Emericellopsis and Parasarocladium, but distinct from all currently known species. Although sharing morphological characteristics with the most closely related species, these genera differ in micromorphological and molecular characters. Thus, three novel species of Emericellopsis (E. cladophorae, sp. nov., E. enteromorphae, sp. nov., and E. phycophila, sp. nov.) and three novel species of Parasarocladium (P. aestuarinum, sp. nov., P. alavariense, sp. nov., and P. fusiforme, sp. nov.) are proposed.

中文翻译：

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与河口环境中的大型藻类相关的 Emericellopsis 和 Parasarocladium 的新型耐盐物种

摘要 Fucus、Ulva 和 Enteromorpha 属的大型藻类通常在河口中很丰富。内生真菌可能对宿主产生有益影响，影响它们应对压力的能力。它们也是生物活性化合物的来源。然而，人们对定居在这些大型藻类中的内生真菌知之甚少。从 Ria de Aveiro（葡萄牙）河口不同地点的大型藻类以及盐水和海绵中获得内生分离物。六个类顶孢属物种不能隶属于任何已知物种。基于核糖体 DNA 的内部转录间隔区 (ITS) 和 β-微管蛋白 (tub2) 和肌动蛋白 (act1) 基因的系统发育分析将这些物种置于 Emericellopsis 和 Parasarocladium 属中，但与所有目前已知的物种不同。尽管与最密切相关的物种共享形态特征，但这些属在微形态和分子特征上有所不同。因此，Emericellopsis 的三个新物种（E. cladophorae, sp. nov., E. enteromorphae, sp. nov. 和 E. phycophila, sp. nov.）和 Parasarocladium 的三个新物种（P. aestuarinum, sp. nov.） ., P. alavariense, sp. nov., 和 P. fusiforme, sp. nov.)。