Sugarcane is an important industrial crop with a high sugar yield that has become a leading energy crop worldwide. It is widely cultivated in tropical and subtropical regions. Various diseases beset the cultivation of sugarcane. The molecular study of disease resistance in sugarcane is limited by its complicated genome. In our study, RNA-seq was employed to detect the mechanism of twisted leaf disease tolerance in modern cultivar sugarcane, which derived from Narenga porphyrocoma. We completed high-throughput transcriptomic sequencing of 12 samples, including three stages of a susceptible (NSBC1_T “H3–8”) and an unsusceptible cultivar (NSBC1_CK “H-19”) with two biological repeats, respectively. Using the Saccharum spontaneum genome as reference, the average mapping ratio of the clean data was over 70%. Among the differentially expressed genes between H3–8 and H3–19, we focused on the analysis of hormone pathways and resistance (R) genes. The results showed that twisted leaf disease triggers hormone networks and around 40% of R genes conditioned lower expression in the susceptible cultivar. One of the possible reasons for H3–8 being susceptible to twisted leaf disease might be the null/retarded response of R genes, especially in pre-onset stage (46% down-regulated) of pathogens infection.

中文翻译：

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Narenga porphyrocoma遗传背景下的易感甘蔗扭曲叶片疾病反应的转录组谱分析

甘蔗是一种重要的工业作物，含糖量高，已成为全球领先的能源作物。在热带和亚热带地区广泛种植。各种疾病困扰着甘蔗的种植。甘蔗抗病性的分子研究受到其复杂基因组的限制。在我们的研究中，RNA-seq用于检测现代栽培甘蔗中的扭曲叶病耐受性机制，该甘蔗源于纳伦加卟啉单胞菌。我们完成了12个样品的高通量转录组测序，分别包括三个阶段的易感品种（NSBC1_T“ H3-8”）和不敏感品种（NSBC1_CK“ H-19”），具有两个生物学重复。使用自发糖精以基因组为参考，纯数据的平均作图率超过70％。在H3–8和H3–19之间的差异表达基因中，我们着重于激素途径和抗性（R）基因的分析。结果表明，扭曲的叶病触发了激素网络，大约40％的R基因限制了易感品种的较低表达。H3-8易患扭曲叶病的可能原因之一可能是R基因的无效应答/迟发应答，尤其是在病原体感染的发病前阶段（下调46％）。