The onion thrips (Thrips tabaci Lindeman, 1889) is a key pest of a wide range of crops because of its ecological attributes such as polyphagy, high reproduction rate, ability to transmit tospoviruses and resistance to insecticides. Recent studies revealed that T. tabaci is a cryptic species complex and it has three lineages (leek-associated arrhenotokous L1-biotype, leek-associated thelytokous L2-biotype and tobacco-associated arrhenotokous T-biotype), however, the adults remain indistinguishable. T. tabaci individuals were collected from different locations of Hungary to create laboratory colonies from each biotypes. Mitochondrial COI (mtCOI) region was sequenced from morphologically identified individuals. After sequence analysis SNPs were identified and used for CAPS marker development, which were suitable for distinguishing the three T. tabaci lineages. Genetic analysis of the T. tabaci species complex based on mtCOI gene confirmed the three well-known biotypes (L1, L2, T) and a new biotype because the new molecular evidence presented in this study suggests T-biotype of T. tabaci forming two distinct (sub)clades (T1 and T2). This genetic finding indicates that the genetic variability of T. tabaci populations is still not fully mapped. We validated our developed marker on thrips individuals from our thrips colonies. The results demonstrated that the new marker effectively identifies the different T. tabaci biotypes. We believe that our reliable genotyping method will be useful in further studies focusing on T. tabaci biotypes and in pest management by scanning the composition of sympatric T. tabaci populations.

中文翻译：

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烟粉虱（Thysanoptera：Thripidae）隐蔽种复合体的简单分子鉴定方法

洋葱蓟马（烟粉虱Lindeman, 1889) 是多种作物的主要害虫，因为它具有多食性、高繁殖率、传播 tospoviruses 的能力和对杀虫剂的抗性等生态特性。最近的研究表明，烟粉虱是一个神秘的物种复合体，它具有三个谱系（韭菜相关的 arrhenotokous L1 生物型、韭菜相关的 thelytokous L2 生物型和烟草相关的 arrhenotokous T 生物型），但是，成年人仍然无法区分。烟粉虱从匈牙利的不同地点收集个体以从每种生物型创建实验室菌落。线粒体 COI (mtCOI) 区域是从形态学鉴定的个体中测序的。序列分析后，SNP 被鉴定并用于 CAPS 标记开发，适用于区分这三种烟粉虱血统。遗传分析烟粉虱基于 mtCOI 基因的物种复合体证实了三种众所周知的生物型（L1、L2、T）和一种新的生物型，因为本研究中提出的新分子证据表明 T 生物型烟粉虱形成两个不同的（子）进化枝（T1 和 T2）。这一遗传发现表明，烟粉虱人口仍然没有完全映射。我们验证了我们在蓟马菌落中的蓟马个体上开发的标记。结果表明，新标记有效地识别了不同的烟粉虱生物型。我们相信我们可靠的基因分型方法将有助于进一步研究烟粉虱生物型和害虫管理通过扫描同域的组成烟粉虱人口。