A 20-day trial was conducted to reveal bacterial community dynamics in a commercial nursery of larval Litopenaeus vannamei larvae. The bacterial communities in the ambient water were profiled by high-throughput sequencing of the V4–V5 hypervariable region of the 16S rRNA gene. The results indicated that the dominant bacterial phyla between the metamorphosis stage and postlarval stage were Bacteroidetes, Proteobacteria, Cyanobacteria, and Firmicutes, representing more than 80.09% of the bacterial operational taxonomic units. The relative abundance among bacterial phyla notably differed between the two stages. The relative abundance of Cyanobacteria was higher in the metamorphosis stage, while that of Bacteroidetes was higher and more stable in the postlarval stage. At the class level, the relative abundance of Sphingobacteriia and Alphaproteobacteria increased markedly in the postlarval stage, while that of Flavobacteriia decreased. Redundancy analysis showed that bacterial composition in the metamorphosis stage was positively correlated with salinity, alkalinity, and pH, while in the postlarval stage, it was positively correlated with ammonium nitrogen and nitrite nitrogen. Thus, microbial community diversity in the nursery phase varies per rearing stage.

中文翻译：

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高通量测序揭示太平洋白对虾（南美白对虾）苗圃饲养过程中的细菌群落动态

进行了为期20天的试验，以揭示凡纳滨对虾幼体的商业苗圃中的细菌群落动态。幼虫。通过对16S rRNA基因的V4-V5高变区进行高通量测序，分析了周围水中的细菌群落。结果表明，在变态阶段和幼虫后阶段之间的优势菌群是拟杆菌，变形杆菌，蓝藻和纤毛虫，占细菌操作分类单位的80.09％以上。在两个阶段之间，细菌门之间的相对丰度明显不同。在变态阶段，蓝细菌的相对丰度较高，而在幼虫后阶段，拟杆菌的相对丰度较高且更稳定。在班级水平上，在幼虫后阶段，Sphingobacteriia和Alphaproteobacteria的相对丰度显着增加，而Flavobacteria则相对减少。冗余度分析表明，变态阶段的细菌组成与盐度，碱度和pH呈正相关，而在幼体阶段，细菌组成与铵态氮和亚硝酸盐氮呈正相关。因此，苗圃阶段的微生物群落多样性在每个饲养阶段都不同。