Backgrounds

Our previous work has confirmed that Hydra magnipapillata extract reduces the toxicity of nanomaterials. We investigated the effects of the Hydra protein (HP) and its role in reducing the toxicity of PVP-coated silver nanoparticles (Ag–PVP NPs) using the A549 lung epithelial cell line.

Results

The viability of cells exposed to Ag–PVP NPs or Ag–PVP NPs + HP was 77.93 ± 6.96% or 95.04 ± 9.20%, respectively, indicating increased viability when HP was added. Although Ag–PVP NPs may have caused apoptosis and necrosis in exposed cells, limited effects were observed in the Ag–PVP NPs + HP exposure group. There were no effects on gene expression between the control and all experimental groups. A549 cell in 3D cultures was affected less by the toxicity of Ag–PVP NPs than those in 2D cultures.

Conclusion

In conclusion, HP appears to be a non-cytotoxic substance and can reduce the cytotoxicity of Ag nanomaterials.

中文翻译：

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九头蛇蛋白可降低3D A549细胞系中Ag-PVP纳米颗粒的毒性

背景资料

我们以前的工作已经证实，九头蛇提取物可降低纳米材料的毒性。我们使用A549肺上皮细胞系研究了Hydra蛋白（HP）的作用及其在降低PVP涂层的银纳米颗粒（Ag–PVP NPs）毒性中的作用。

结果

暴露于Ag-PVP NPs或Ag-PVP NPs + HP的细胞的活力分别为77.93±6.96％或95.04±9.20％，表明添加HP时活力增强。尽管Ag-PVP NPs可能引起暴露细胞的凋亡和坏死，但在Ag-PVP NPs + HP暴露组中观察到的作用有限。在对照组和所有实验组之间对基因表达没有影响。3D培养物中的A549细胞受Ag–PVP NPs的毒性的影响要小于2D培养物中的A549细胞。

结论

总之，HP似乎是一种非细胞毒性物质，可以降低Ag纳米材料的细胞毒性。