An incubation experiment with equivalent N rates was conducted for 56 days using a typical black soil amended with chemical fertilizer with or without straw amendment using a 15N cross-labeling technique. Compared with the chemical fertilizer treatment (15NCF), chemical fertilizer combined with straw treatment (CF + S) showed a significantly higher (P < 0.05) contribution from applied N to microbial biomass N (BN) in the first 14 days and to particulate organic N (PON) and mineral-associated total N (MON) throughout the incubation. Straw application in the CF + S treatment significantly (P < 0.05) decreased the recovery of chemical fertilizer N as soil inorganic N except at day 3 but increased the recovery of chemical fertilizer N as BN before day 14 and as PON and MON from day 14 to the end of the incubation period. At the end of the incubation period, the total N2O-N emissions in the CF + S treatment increased significantly (P < 0.05) compared with the CF treatment, and the increase in N2O-N emissions was 73% from chemical fertilizer and 27% from straw N individually. Our results suggest that the combined application of chemical fertilizer and straw increased soil fertility together with an increase in N2O emissions in the typical black soil and the N2O emissions from straw cannot be ignored.

中文翻译：

---

对典型黑钙土施施化肥和秸秆后土壤氮库和 N2O 排放的量化

使用 15N 交叉标记技术，使用添加或不添加秸秆添加或不添加秸秆添加化学肥料的典型黑土进行了 56 天等效氮肥的孵化实验。与化肥处理（15NCF）相比，化肥结合秸秆处理（CF + S）在前 14 d 显示出显着更高（P < 0.05）施氮对微生物生物量 N（BN）和颗粒有机物的贡献N (PON) 和矿物相关的总 N (MON) 在整个孵化过程中。CF + S 处理中秸秆施用显着（P < 0.05）降低了化肥 N 作为土壤无机 N 的回收率，但在第 3 天除外，但在第 14 天前以 BN 和 PON 和 MON 从第 14 天增加了化肥 N 的回收率到潜伏期结束。潜伏期结束时，CF+S处理的N2O-N总排放量较CF处理显着增加（P<0.05），N2O-N排放增加量分别为化肥的73%和27%从稻草 N 单独。我们的研究结果表明，化肥和秸秆复合施用增加了土壤肥力，同时典型黑土中 N2O 排放量增加，秸秆中的 N2O 排放量不容忽视。