Abstract

The residual elimination of altrenogest in swine was investigated, preparing for the determination of withdrawal time. The residues of altrenogest in sebum, muscle, liver and kidney were extracted by optimized methods and further analyzed by UPLC–MS/MS. Under experimental conditions, the LOD and LOQ of altrenogest in sebum, muscle, liver and kidney were 0.5 and 1.0 μg/kg, respectively. The recoveries were in the range of 65 and 95% and the inter- and intra-RSD were less than 15%. The established method for the extraction, purification and detection of altrenogest is suitable for the determination of the residue of altrenogest in edible tissues of pigs. It was showed that altrenogest had the highest residual concentration level in liver, followed by kidney, sebum and muscle. Then withdrawal time was set at 9 days. The study provides an effective basis for elimination of altrenogest in swine.

摘要

研究了猪体内altrenogest的残留消除，为停药时间的确定做准备。采用优化方法提取皮脂、肌肉、肝脏和肾脏中的altenogest残留，并通过UPLC-MS/MS进一步分析。在实验条件下，altenogest 在皮脂、肌肉、肝脏和肾脏中的 LOD 和 LOQ 分别为 0.5 和 1.0 μg/kg。回收率在 65% 和 95% 之间，并且内部和内部 RSD 小于 15%。所建立的altrenogest提取纯化检测方法适用于猪可食组织中altrenogest残留量的测定。结果表明，altrenogest在肝脏中的残留浓度最高，其次是肾脏、皮脂和肌肉。然后取款时间定为9天。