Abstract

The production of microbial protein in the form of yeast grown on lignocellulosic sugars and nitrogen-rich industrial residues is an attractive approach for reducing dependency on animal and plant protein. Growth media composed of enzymatically saccharified sulfite-pulped spruce wood, enzymatic hydrolysates of poultry by-products and urea were used for the production of single-cell protein. Strains of three different yeast species, Cyberlindnera jadinii, Wickerhamomyces anomalus and Blastobotrys adeninivorans, were cultivated aerobically using repeated fed-batch fermentation up to 25 L scale. Wickerhamomyces anomalus was the most efficient yeast with yields of 0.6 g of cell dry weight and 0.3 g of protein per gram of glucose, with cell and protein productivities of 3.92 g/L/h and 1.87 g/L/h, respectively. Using the conditions developed here for producing W. anomalus, it would take 25 industrial (200 m³) continuously operated fermenters to replace 10% of the fish feed protein used in Norway.

中文翻译：

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在反复补料分批发酵过程中，以云杉糖和家禽水解产物为生长培养基，以生产酵母生物质

摘要

以木质纤维素和富含氮的工业残基生长的酵母形式生产微生物蛋白是减少对动植物蛋白依赖性的一种有吸引力的方法。由酶促糖化亚硫酸盐制的云杉木材，家禽副产物的酶解产物和尿素组成的生长培养基用于生产单细胞蛋白。使用重复补料分批发酵法，在不超过25 L规模的条件下，有氧地培养三种不同酵母菌种，即Cyber​​berdnera jadinii，Wickerhamomyces anomalus和Blastobotrys adeninivorans。柳条柳条是最有效的酵母，每克葡萄糖的干重为0.6 g，蛋白质的产量为0.3 g，细胞和蛋白质的生产率分别为3.92 g / L / h和1.87 g / L / h。使用此处开发的生产异常W.的条件，将需要25个工业（200 m ³）连续操作的发酵罐来替代挪威使用的10％的鱼饲料蛋白。