Cell-free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices.,Biotechnology and Bioengineering

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Cell-free protein synthesis: The transition from batch reactions to minimal cells and microfluidic devices.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-01-30 , DOI: 10.1002/bit.27248
Mohammad H Ayoubi-Joshaghani ₁ , Hassan Dianat-Moghadam ₂ , Khaled Seidi ₃ , Ali Jahanban-Esfahalan ₄ , Peyman Zare ₅ , Rana Jahanban-Esfahlan _{6,

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Affiliation

Thanks to the synthetic biology, the laborious and restrictive procedure for producing a target protein in living microorganisms by biotechnological approaches can now experience a robust, pliant yet efficient alternative. The new system combined with lab‐on‐chip microfluidic devices and nanotechnology offers a tremendous potential envisioning novel cell‐free formats such as DNA brushes, hydrogels, vesicular particles, droplets, as well as solid surfaces. Acting as robust microreactors/microcompartments/minimal cells, the new platforms can be tuned to perform various tasks in a parallel and integrated manner encompassing gene expression, protein synthesis, purification, detection, and finally enabling cell‐cell signaling to bring a collective cell behavior, such as directing differentiation process, characteristics of higher order entities, and beyond. In this review, we issue an update on recent cell‐free protein synthesis (CFPS) formats. Furthermore, the latest advances and applications of CFPS for synthetic biology and biotechnology are highlighted. In the end, contemporary challenges and future opportunities of CFPS systems are discussed.

中文翻译：

无细胞蛋白质合成：从批反应到最小细胞和微流控设备的过渡。

得益于合成生物学，现在通过生物技术方法在活微生物中生产目标蛋白的费力且限制性的程序现在可以体验到健壮，灵活而有效的替代方法。新系统与芯片实验室微流体设备和纳米技术相结合，为构想新型无细胞格式（例如DNA刷，水凝胶，囊泡颗粒，液滴以及固体表面）提供了巨大的潜力。新平台可充当强大的微反应器/微区室/最小细胞，可以进行调整以并行和集成的方式执行各种任务，包括基因表达，蛋白质合成，纯化，检测，并最终使细胞信号传导带来集体细胞行为，例如指导微分过程，高阶实体的特征，超越。在这篇综述中，我们发布了有关最新的无细胞蛋白质合成（CFPS）格式的更新。此外，重点介绍了CFPS在合成生物学和生物技术方面的最新进展和应用。最后，讨论了CFPS系统的当代挑战和未来机遇。

更新日期：2020-03-09

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