This study presents an easy paper-plastic hybrid microfluidic chip fabrication method that explored the use of oven baking a wax-printed pattern for microfluidic channel assembly combined with the use of a double-sided adhesive tape for layer binding. More specifically, the method includes printing of a wax pattern onto the CA film & oven baking of the pattern along with the backed NC membrane at 120°C for 2.5 minutes. The porous nature of the NC membrane allows the wax pattern to enter into its pores during the baking process and binds the backed NC membrane with the CA film. Attachment of the engraved PMMA layer was performed to increase the structural support. The engraved hole of the layer allows the conjugate pad with space for its integration into the system. Moreover, the antibodies required for the integration of the chip with the LFIA were selected by enzyme-linked immunosorbent assay (ELISA). A CA film, an NC membrane, and a PMMA sheet were used to develop a microfluidic chip that can be integrated with a sandwich format lateral flow immunoassay used to detect dengue non-structural 1 protein. The fabricated chip was able to successfully detect a concentration of dengue non-structural 1 protein of at least 84.66 ng/mL.

中文翻译：

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新型纸塑微流混合芯片与侧向流免疫测定相结合，用于登革热非结构蛋白1抗原检测。

这项研究提出了一种简单的纸塑混合微流控芯片制造方法，该方法探索了如何使用烤箱烘烤蜡印刷的图案进行微流控通道组装，以及如何使用双面胶带进行层粘合。更具体地，该方法包括将蜡图案印刷到CA膜上并在120℃下对该图案连同背衬的NC膜进行烤箱烘烤2.5分钟。NC膜的多孔性质使蜡模在烘烤过程中进入其孔中，并使背衬的NC膜与CA膜粘合在一起。进行雕刻的PMMA层的附着以增加结构支撑。该层的雕刻孔允许共轭垫具有将其集成到系统中的空间。此外，通过酶联免疫吸附试验（ELISA）选择芯片与LFIA整合所需的抗体。使用CA膜，NC膜和PMMA片来开发微流控芯片，该芯片可与用于检测登革热非结构性1蛋白的夹心式横向流免疫分析法集成。制成的芯片能够成功检测到至少84.66 ng / mL的登革热非结构性1蛋白浓度。