Different bacterial isolates were obtained from a much-polluted lake (Lake Mariout) in Alexandria, Egypt. They were tested to bio-remove different heavy metal ions (Cu2+, Pb2+, Cd2+, Co2+, Ni2+, Zn2+, Mn2+, and Fe3+). In addition, this study was aimed to create a fixed bed column to enhance the metal removal from some polluted wastewater samples. The potent bacterium was selected and identified as Pseudomonas aeruginosa using the 16SrRNA gene sequence. The effect of some physicochemical parameters on the bio-removal process was studied in batch cultures; it was found the efficiency % of metal removal was increased on using pH 7.5 and bacterial biomass of 750 mg/l. Also, the use of the fixed bed column led to an increase in the removal efficiency % to 100% for the Cu2+, Zn2+, and Cd2+ ions and decrease the consuming time from 48 to 24 h under using the optimum incubation conditions, while the removal of Fe3+ and Pb2+ showed 62% and 47%, respectively, with a 20% increase compared to the batch system. It was confirmed the use of fixed bed bioreactors was able to increase the efficiency towards the metal removal in polluted environmental samples while decreasing the exhaustion time. Also, Pseudomonas sp. showed great ability to get rid of many harmful health hazard substances.

中文翻译：

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使用装有固体载体的固定床生物反应器去除重金属

从埃及亚历山大的一个污染严重的湖泊（马里奥湖）中获得了不同的细菌分离物。测试它们可以生物去除不同的重金属离子（Cu2+、Pb2+、Cd2+、Co2+、Ni2+、Zn2+、Mn2+ 和 Fe3+）。此外，本研究旨在创建一个固定床柱，以提高一些污染废水样品中的金属去除率。使用 16SrRNA 基因序列选择并鉴定有效细菌为铜绿假单胞菌。在分批培养中研究了一些理化参数对生物去除过程的影响；发现使用 pH 值 7.5 和细菌生物量 750 mg/l 时，金属去除效率 % 增加。此外，固定床塔的使用导致 Cu2+、Zn2+、和 Cd2+ 离子，并在使用最佳孵育条件下将消耗时间从 48 小时减少到 24 小时，而 Fe3+ 和 Pb2+ 的去除率分别显示 62% 和 47%，与批处理系统相比增加了 20%。经证实，固定床生物反应器的使用能够提高污染环境样品中金属去除的效率，同时减少消耗时间。此外，假单胞菌属。显示出去除许多有害健康危害物质的强大能力。假单胞菌属 显示出去除许多有害健康危害物质的强大能力。假单胞菌属 显示出去除许多有害健康危害物质的强大能力。